Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23179
Title: Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge
Authors: Assis, Andrêssa S. F.
Otenio, Marcelo Henrique
Drumond, Betânia P.
Fumian, Tulio M.
Miagostovich, Marize P.
Silva, Maria Luzia da Rosa e
Affilliation: Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil.
Embrapa Gado de Leite. Juiz de Fora, MG, Brasil.
Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil.
Abstract: Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge samples was carried out. Different types of sludge were obtained in two WWTPs and processed using protocol C. HAdV was detected in all samples, with a similar or higher viral load than those obtained with other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.
Keywords: Sewage sludge
Wastewater treatment plant
Organic flocculation
Human adenovirus
Internal control
Quantitative PCR
keywords: Reação em Cadeia da Polimerase em Tempo Real
Adenovírus Humanos
Floculação orgânica
Estação de tratamento de águas residuais
Controle interno
Lodo de esgoto
Issue Date: 2017
Publisher: Elsevier
Citation: ASSIS, Andrêssa S. F. et al. Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. Science of the Total Environment, n.583, p.163-168, 2017.
DOI: 10.1016/j.scitotenv.2017.01.045
ISSN: 0048-9697
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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