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MOLECULAR MECHANISMS OF THERMAL RESISTANCE OF THE INSECT TRYPANOSOMATID CRITHIDIA THERMOPHILA
Ishemgulova, Aygul et al. | Date Issued: 2017
Author
Ishemgulova, Aygul
Butenko, Anzhelika
Kortišová, Lucie
Boucinha, Carolina
Grybchuk-Ieremenko, Anastasiia
Morelli, Karina A.
Tesařová, Martina
Kraeva, Natalya
Grybchuk, Danyil
Pánek, Tomáš
Flegontov, Pavel
Lukeš, Julius
Votýpka, Jan
Pavan, Márcio Galvão
Opperdoes, Fred R.
Spodareva, Viktoria
Levy, Claudia M. d'Avila
Kostygov, Alexei Yu
Yurchenko, Vyacheslav
Affilliation
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários. Rio de Janeiro, RJ. Brasil.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários. Rio de Janeiro, RJ. Brasil / Universidade Estadual do Rio de Janeiro. Instituto de Biologia Roberto Alcãntara Gomes. Departamento de Ecologia. Rio de Janeiro, RJ, Brasil.
Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic / Russian Academy of Sciences. Institute for Information Transmission Problems. Moscow, Russia.
Czech Academy of Sciences. Biology Centre. Institute of Parasitology. České Budějovice (Budweis), Czech Republic. / University of South Bohemia. Faculty of Sciences. České Budějovice (Budweis), Czech Republic / Canadian Institute for Advanced Research.Toronto, Ontario, Canada.
Czech Academy of Sciences. Biology Centre. Institute of Parasitology. České Budějovice (Budweis), Czech Republic. / Charles University / Faculty of Science. Department of Parasitology. Prague, Czech Republic.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
Université Catholique de Louvain. de Duve Institute. Brussels, Belgium.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Zoological Institute of the Russian Academy of Sciences. St. Petersburg, Russia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários. Rio de Janeiro, RJ. Brasil..
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Zoological Institute of the Russian Academy of Sciences. St. Petersburg, Russia.
University of Ostrava. Faculty of Science. Life Science Research Centre. Ostrava, Czech Republic / Czech Academy of Sciences. Biology Centre. Institute of Parasitology. Česke Budějovice (Budweis), Czech Republic / Albert Einstein College of Medicine. Department of Pathology. New York, NY, USA / University of Ostrava. Faculty of Science. Institute of Environmental Technologies. Ostrava. Czech Republic.
Abstract
In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which we reclassified as a separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles of flagellates cultivated at low (23°C) and elevated (34°C) temperature. When maintained at high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism and mitochondrial stress protection significantly upregulated. Comparison with another thermoresistant monoxenous trypanosomatid, Leptomonas seymouri, revealed dramatic differences in transcription profiles of the two species with only few genes showing the same expression pattern. This disparity illustrates differences in the biology of these two parasites and distinct mechanisms of their thermotolerance, a prerequisite for living in warm-blooded vertebrates.
Keywords in Portuguese
Crithidia
Mecanismos moleculares
resistência térmica
 
Keywords
Crithidia luciliae thermophila
Molecular mechanisms
thermal resistance
 
Publisher
Public Library of Science
Citation
ISHEMGULOVA, Aygul et al. Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila. Plos One, v.12, n.3, e0174165, Mar. 2017.
DOI
10.1371/journal.pone.0174165
ISSN
1932-6203