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https://www.arca.fiocruz.br/handle/icict/23267
DYNAMICS OF CRISPR LOCI IN MICROEVOLUTIONARY PROCESS OF YERSINIA PESTIS STRAINS
DNA Intergênico
Análise de Sequência de DNA
Genoma Bacteriano
Genética
Yersinia pseudotuberculosis
DNA, Intergenic
Yersinia pseudotuberculosis
Sequence Analysis, DNA
Genome, Bacterial
Genetics
genética
Sequência de Bases
Brasil
Variação Genética
Dados de Sequência Molecular
Yersinia pestis
classificação
Genótipo
Filogenia
Peste
microbiologia
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Abstract
The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution of 146 Yersinia pestis strains from different biovars and locations was investigated in this work. The majority of strains from the Orientalis biovar presented specific spacer arrays, allowing for the establishment of a CRISPR signature for their respective isolates. Twenty-one new spacers were found in the Y. pestis strains from plague foci in Brazil. Ninety-three (64%) strains were grouped in the G1 genotype, whereas the others were distributed in 35 genotypes. This study allowed observing a microevolutionary process in a group of Y. pestis isolated from Brazil. We also identified specific genotypes of Y. pestis that were important for the establishment of the bacteria in plague foci in Brazil. The data have provided supporting evidence for the diversity and dynamics of CRISPR loci present in the genome of Y. pestis strains from plague foci in Brazil.
Keywords in Portuguese
DNA BacterianoDNA Intergênico
Análise de Sequência de DNA
Genoma Bacteriano
Genética
Yersinia pseudotuberculosis
Keywords
DNA, BacterialDNA, Intergenic
Yersinia pseudotuberculosis
Sequence Analysis, DNA
Genome, Bacterial
Genetics
DeCS
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadasgenética
Sequência de Bases
Brasil
Variação Genética
Dados de Sequência Molecular
Yersinia pestis
classificação
Genótipo
Filogenia
Peste
microbiologia
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