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DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR-BASED ASSAYS FOR ESTIMATION OF NATURAL INFECTION RATES AND TRYPANOSOMA CRUZI LOAD IN TRIATOMINE VECTORS
Reação em Cadeia da Polimerase Multiplex
Reação em Cadeia da Polimerase em Tempo Real
Triatominae
Carga Parasitária
Infecção natural
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Abstract
Chagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. The diversity of vector species and their habitats are among the factors that hinder the control of the disease. Control programs periodically monitor the prevalence of T. cruzi infection in insect bugs through microscopical observation of diluted feces. However, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all evolutionary stages of the insect and may in turn be limited to differentiate T. cruzi from other morphologically similar trypanosomatids. Here, we report two highly sensitive and accurate methodologies to infer T. cruzi infection rates and to quantify parasite load in the gut of field-collected triatomines.
Keywords in Portuguese
Trypanosoma cruziReação em Cadeia da Polimerase Multiplex
Reação em Cadeia da Polimerase em Tempo Real
Triatominae
Carga Parasitária
Infecção natural
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