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PROTEOMIC ANALYSIS OF THE SECRETOME OF HEPG2 CELLS INDICATES DIFFERENTIAL PROTEOLYTIC PROCESSING AFTER INFECTION WITH DENGUE VIRUS
Caruso, Marjolly B. et al. | Date Issued: 2017
Author
Caruso, Marjolly B.
Trugilho, Monique R. O.
Higa, Luiza M.
Ferreira, André S. Teixeira
Perales, Jonas
Poian, Andrea T. Da
Zingali, Russolina B.
Affilliation
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil
Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil
Abstract
Secretome analysis can be described as a subset of proteomics studies consisting in the analysis of the molecules secreted by cells or tissues. Dengue virus (DENV) infection can lead to a broad spectrum of clinical manifestations, with the severe forms of the disease characterized by hemostasis abnormalities and liver injury. The hepatocytes are a relevant site of viral replication and a major source of plasma proteins. Until now, we had limited information on the small molecules secreted by hepatic cells after infection by DENV. In the present study, we analysed a fraction of the secretome of mock- and DENV-infected hepatic cells (HepG2 cells) containing molecules with <10kDa, using different proteomic approaches. We identified 175 proteins, with 57 detected only in the samples from mock-infected cells, 59 only in samples from DENV-infected cells, and 59 in both conditions. Most of the peptides identified were derived from proteins larger than 10kDa, suggesting a proteolytic processing of the secreted molecules. Using in silico analysis, we predicted consistent differences between the proteolytic processing occurring in mock and DENV-infected samples, raising, for the first time, the hypothesis that differential proteolysis of secreted molecules would be involved in the pathogenesis of dengue.
Keywords in Portuguese
Vírus da Dengue
Hepatócitos
Proteoma
Proteínas de baixa massa molecular
Predição de enzimas proteolíticas
 
Keywords
Dengue virus
Hepatic cells
Proteome
Low molecular mass proteins
Prediction of proteolytic enzymes
Secretome
 
Publisher
1876-7737
Citation
CARUSO, Marjorlly B. et al. Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus. Journal of Proteomics, v.151, p.106-113, July 2017.
DOI
10.1016/j.jprot.2016.07.011
ISSN
1874-3919