Introduction: An important factor that contributes to the severity of Chagas disease in people living in endemic areas is the occurrence of multiple infections with Trypanosoma cruzi. Experimental studies have shown the increased morbidity due to successive reinfections with different strains, with the possibility of to evaluate the influence of multiple infections upon the tissue lesions. Multiple infections could also influence an activation of the disease in patients with HIV infection (AIDs) or immunosuppressed in cases of organs transplantation. In such cases, a reactivation of virulent strains could occur with aggravation of the disease. Objective: to evaluate the immunopathological response in mice triple infected with different T. cruzi strains and treated with immunosuppressor drugs.