Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23640
Title: Development of duplex-PCR for identification of Aeromonas species
Authors: Marques, Carina Lucena Mendes
Hofer, Ernesto
Leal, Nilma Cintra
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Abstract: Introduction: The number of reports of intestinal infections caused by Aeromonas spp. has increased signifi cantly in recent years. In most clinical laboratories, identifi cation of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vibrio. Methods: A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets identifying Aeromonas spp. pathogenic to humans. Results: The duplex-PCR results were reproducible and specifi c for Aeromonas spp. pathogenic to humans. Conclusions: This method will allow differentiation between Vibrio and Aeromonas spp. in patients with in cholera-like symptoms and can also be used in water quality monitoring.
Keywords: Aeromonas
Identification
PCR
keywords: Aeromonas
Identificação
PCR
DeCS: Técnicas de Tipagem Bacteriana
Humanos
Aeromonas
Genética
Diarreia
Microbiologia
Infecções por Bactérias Gram-Negativas
Microbiologia
Reação em Cadeia da Polimerase
Métodos
Aeromonas
Isolamento & purificação
Vibrio
Genética
Vibrio
Isolamento & purificação
Reprodutibilidade dos Testes
Issue Date: 2013
Citation: MARQUES, Carina Lucena Mendes; HOFER, Ernesto; LEAL, Nilma Cintra. Development of duplex-PCR for identification of Aeromonas species. Revista Da Sociedade Brasileira De Medicina Tropical, v. 46, n. 3, p. 355–357, May/Jun. 2013.
DOI: 10.1590/0037-8682-1344-2013
ISSN: 1678-9849
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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