Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23654
Title: Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
Authors: Morais, Rayana Carla Silva de
Gonçalves-de-Albuquerque, Suênia da Cunha
Pessoa e Silva, Rômulo
Costa, Pietra Lemos
Silva, Kamila Gaudêncio da
Silva, Fernando José da
Brandão-Filho, Sinval Pinto
Dantas-Torres, Filipe
Paiva-Cavalcanti, Milena de
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / Federal Rural University of Pernambuco. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Federal Rural University of Pernambuco. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / University of Bari. Department of Veterinary Medicine. Bari, Italy.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Abstract: American cutaneous leishmaniasis (ACL) is a disease caused by different species of Leishmania protozoa, Leishmania braziliensis being the main species found in Brazil. In this study, two rural areas in Pernambuco, northeastern Brazil, where ACL is endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) and tested using a conventional PCR and a quantitative real time PCR. A total of 117 ectoparasites were collected, being 50 (42.74%) of them positive for L. braziliensis (in at least one PCR protocol), with a mean parasite load of 14.14 fg/μL. Furthermore, 46 (92.00%) positive ectoparasites were collected from positive dogs and 4 (8.00%) from negative ones. This study reports the detection of L. braziliensis DNA in ectoparasites, but does not prove their vector competence. Certainly, experimental transmission studies are necessary to assess their role, if any, in the transmission of Leishmania parasites to dogs.
Keywords: Ectoparasites
Dogs
Leishmania braziliensis
PCR
Real time PCR
keywords: Ectoparasitas
Cães
Leishmania braziliensis
PCR
PCR em tempo real
DeCS: Doenças do Cão
parasitologia
Leishmania braziliensis
isolamento & purificação
Ftirápteros
parasitologia
Sifonápteros
parasitologia
Carrapatos
parasitologia
Animais
Cães
DNA de Protozoário
isolamento & purificação
Ectoparasitoses
parasitologia
Ectoparasitoses
veterinária
Issue Date: 2013
Citation: MORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.
DOI: 10.1016/j.vetpar.2013.03.026
ISSN: 1873-2550
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos

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