Title:	Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
Authors:	Morais, Rayana Carla Silva de Gonçalves-de-Albuquerque, Suênia da Cunha Pessoa e Silva, Rômulo Costa, Pietra Lemos Silva, Kamila Gaudêncio da Silva, Fernando José da Brandão-Filho, Sinval Pinto Dantas-Torres, Filipe Paiva-Cavalcanti, Milena de
Affilliation:	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / Federal Rural University of Pernambuco. Recife, PE, Brazil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil. Federal Rural University of Pernambuco. Recife, PE, Brazil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / University of Bari. Department of Veterinary Medicine. Bari, Italy. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Abstract:	American cutaneous leishmaniasis (ACL) is a disease caused by different species of Leishmania protozoa, Leishmania braziliensis being the main species found in Brazil. In this study, two rural areas in Pernambuco, northeastern Brazil, where ACL is endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) and tested using a conventional PCR and a quantitative real time PCR. A total of 117 ectoparasites were collected, being 50 (42.74%) of them positive for L. braziliensis (in at least one PCR protocol), with a mean parasite load of 14.14 fg/μL. Furthermore, 46 (92.00%) positive ectoparasites were collected from positive dogs and 4 (8.00%) from negative ones. This study reports the detection of L. braziliensis DNA in ectoparasites, but does not prove their vector competence. Certainly, experimental transmission studies are necessary to assess their role, if any, in the transmission of Leishmania parasites to dogs.
Keywords:	Ectoparasites Dogs Leishmania braziliensis PCR Real time PCR
keywords:	Ectoparasitas Cães Leishmania braziliensis PCR PCR em tempo real
DeCS:	Doenças do Cão parasitologia Leishmania braziliensis isolamento & purificação Ftirápteros parasitologia Sifonápteros parasitologia Carrapatos parasitologia Animais Cães DNA de Protozoário isolamento & purificação Ectoparasitoses parasitologia Ectoparasitoses veterinária
Issue Date:	2013
Citation:	MORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.
DOI:	10.1016/j.vetpar.2013.03.026
ISSN:	1873-2550
Copyright:	open access
Appears in Collections:	PE - IAM - Artigos de Periódicos

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