Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23666
Title: Cytokines and NO in American tegumentary leishmaniasis patients: profiles in active disease, after therapy and in self-healed individuals
Authors: Souza, Marina de Assis
Castro, Maria C. A. Brelaz de
Oliveira, Andresa Pereira de
Almeida, Amanda Ferreira de
Almeida, Thays Miranda de
Reis, Luiza C.
Medeiros, Ângela Cristina Rapela
Brito, Maria Edileuza Felinto de
Pereira, Valéria Rêgo Alves
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Universidade de São Paulo (IMT/USP). Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil.
Universidade de Pernambuco (HUOC/UPE). Hospital Universitário Oswaldo Cruz. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.
Abstract: Studies suggest the influence of immune response on the successful treatment of American tegumentary leishmaniasis (ATL), and indicate the existence of protective immunity in self-healed patients. Thus, the aim of this work was to quantify interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin (IL-) 10, IL-17, IL-22 and nitric oxide (NO) in culture supernatants of PBMC from patients with active disease (AD), after treatment (AT), and from self-healed (SH) and healthy subjects (CT), in response to Leishmania (Viannia) braziliensis insoluble antigen (AgIns). All groups of patients produced IFN-γ, indicating a predominant proinflammatory profile. AD and AT patients presented TNF-α levels, with a slight increase after therapy, whereas it was weakly quantified in SH. Interestingly, NO secretion was significant in these individuals, whereas IL-17 appeared in low levels and seems to be regulated by NO. Although IL-22 was detected in AD, its role is still questionable. The presence of IL-10 in all groups of patients suggests that the cytokine plays distinct roles in the disease. These results indicate that specific cellular immunity takes part against Leishmania, but with some similarities between the different clinical states herein described; these mediators seem to be necessary for the cure to occur.
Keywords: Cytokines
Cellular immunity
Leishmania braziliensis
Leishmaniasis
Nitric oxide
keywords: Citocinas
Imunidade celular
Leishmania braziliensis
Leishmaniose
Óxido nítrico
DeCS: Citocinas
biossíntese
Leishmania braziliensis
imunologia
Leishmaniose Cutânea
imunologia
Leishmaniose Cutânea
metabolismo
Óxido Nítrico
biossíntese
Adulto
Idoso
Humanos
Idoso de 80 Anos ou mais
Antígenos de Protozoários
imunologia
Citocinas
imunologia
Feminino
Masculino
Leucócitos Mononucleares
imunologia
Leucócitos Mononucleares
metabolismo
Meia-Idade
Adulto Jovem
Issue Date: 2013
Publisher: Elsevier
Citation: SOUZA, M. de Assis et al. Cytokines and NO in American tegumentary leishmaniasis patients: profiles in active disease, after therapy and in self-healed individuals. Microbial Pathogenesis, v. 57, p. 27–32, abr. 2013.
DOI: 10.1016/j.micpath.2013.02.004
ISSN: 1096-1208
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
ART. Cytokines and NO - SOUZA.pdf319.83 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.