| Author | Silva, Everton Fagonde da | |
| Author | Medeiros, Marco Alberto | |
| Author | McBride, Alan John Alexander | |
| Author | Matsunaga, Jim | |
| Author | Esteves, Gabriela dos Santos | |
| Author | Ramos, João Gabriel Rosa | |
| Author | Santos, Cleiton Silva | |
| Author | Croda, Júlio Henrique Rosa | |
| Author | Homma, Akira | |
| Author | Dellagostin, Odir Antonio | |
| Author | Haake, David A. | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Ko, Albert Icksang | |
| Access date | 2017-12-22T16:52:56Z | |
| Available date | 2017-12-22T16:52:56Z | |
| Document date | 2007 | |
| Citation | SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007. | pt_BR |
| ISSN | 0264-410X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23765 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Previous version | https://www.arca.fiocruz.br/handle/icict/5818 | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leptospirose | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Subject in Portuguese | Proteína recombinante | pt_BR |
| Subject in Portuguese | Imunidade | pt_BR |
| Subject in Portuguese | Anticorpos | pt_BR |
| Subject in Portuguese | Hamsters | pt_BR |
| Title | The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.vaccine.2007.05.053 | |
| Abstract | Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil. | pt_BR |
| Affilliation | Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. NY, USA. | pt_BR |
| Subject | Leptospirosis | pt_BR |
| Subject | Subunit vaccine | pt_BR |
| Subject | Leptospiral immunoglobulin-like protein | pt_BR |
| Subject | Recombinant protein | pt_BR |
| Subject | Immunity | pt_BR |
| Subject | Antibodies | pt_BR |
| Subject | Hamsters | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
