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THE TERMINAL PORTION OF LEPTOSPIRAL IMMUNOGLOBULIN-LIKE PROTEIN LIGA CONFERS PROTECTIVE IMMUNITY AGAINST LETHAL INFECTION IN THE HAMSTER MODEL OF LEPTOSPIROSIS
Silva, Everton Fagonde da et al. | Date Issued: 2007
Author
Silva, Everton Fagonde da
Medeiros, Marco Alberto
McBride, Alan John Alexander
Matsunaga, Jim
Esteves, Gabriela dos Santos
Ramos, João Gabriel Rosa
Santos, Cleiton Silva
Croda, Júlio Henrique Rosa
Homma, Akira
Dellagostin, Odir Antonio
Haake, David A.
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. NY, USA.
Abstract
Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis.
Keywords in Portuguese
Leptospirose
Vacina
Proteína recombinante
Imunidade
Anticorpos
Hamsters
 
Keywords
Leptospirosis
Subunit vaccine
Leptospiral immunoglobulin-like protein
Recombinant protein
Immunity
Antibodies
Hamsters
 
Publisher
Elsevier
Citation
SILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007.
Previous version
https://www.arca.fiocruz.br/handle/icict/5818
DOI
10.1016/j.vaccine.2007.05.053
ISSN
0264-410X