| Author | Nicol, Alcina F. | |
| Author | Brunette, Laurie L. | |
| Author | Nuovo, Gerard J. | |
| Author | Grinsztejn, Beatriz | |
| Author | Friedman, Ruth K. | |
| Author | Veloso, Valdiléa G. | |
| Author | Cunha, Cynthia B. | |
| Author | Coutinho, José R. | |
| Author | Andrade, Cecilia Vianna de | |
| Author | Oliveira, Nathalia S. | |
| Author | Woodham, Andrew W. | |
| Author | Silva, Diana M. da | |
| Author | Kast1, W. Martin | |
| Access date | 2018-01-02T11:59:18Z | |
| Available date | 2018-01-02T11:59:18Z | |
| Document date | 2016 | |
| Citation | NICOL, Alcina F. et al. Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients. J Acquir Immune Defic Syndr., v.73, n.1, p.27–33, Sept. 2016. | pt_BR |
| ISSN | 1525-4135 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23840 | |
| Language | eng | pt_BR |
| Publisher | Lippincott, Williams & Wilkins | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | HIV | pt_BR |
| Subject in Portuguese | Inibidor Secretado de Peptidases Leucocitárias | pt_BR |
| Subject in Portuguese | Papillomaviridae | pt_BR |
| Subject in Portuguese | Neoplasias | pt_BR |
| Title | Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients | pt_BR |
| Type | Article | |
| Abstract | Objective: The aim of this study was to evaluate secretory leukocyte
protease inhibitor (SLPI) expression in anal biopsies from HIVpositive
(HIV+) individuals, and compare that to anal intraepithelial
neoplasia (AIN) diagnoses and human papillomavirus (HPV) status.
Design: This is a cross-sectional study of a cohort of 54 HIV+ (31
males and 23 females) from an AIDS clinic in Rio de Janeiro, Brazil.
Methods: The study material consisted of anorectal tissue biopsies
obtained from HIV+ subjects, which were used to construct tissue
microarray paraffin blocks for immunohistochemical analysis of SLPI
expression. Biopsies were evaluated by an expert pathologist and
classified as low-grade AIN1, high-grade AIN2/3, or normal squamous
epithelium. In addition, DNA from the biopsies was extracted
and analyzed for the presence of low- or high-risk HPV DNA.
Results: Histologically, normal squamous epithelium from the
anorectal region showed strong positive SLPI staining in 17/20
(85%) samples. In comparison, 9/17 (53%) dysplastic squamous
epithelial samples from AIN1 patients showed strong SLPI staining,
and only 5/17 (29%) samples from AIN2/3 patients exhibited strong
SPLI staining, which both were significantly fewer than those from
normal tissue (P = 0.005). Furthermore, there was a significantly
higher proportion of samples in which oncogenic high-risk HPV
genotypes were detected in low SLPI-expressing tissues than that in
tissues with high SLPI expression (P = 0.040).
Conclusions: Taken together these results suggest that low SLPI
expression is associated with high-risk HPV infections in the
development of AIN. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | Ohio State University. Comprehensive Cancer Center. Columbus, OH, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Patologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA. | pt_BR |
| Affilliation | University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA. | pt_BR |
| Subject | secretory leukocyte protease inhibitor, | pt_BR |
| Subject | anal intraepithelial neoplasia | pt_BR |
| Subject | human immunodeficiency virus | pt_BR |
| Subject | human papillomavirus | pt_BR |
| e-ISSN | 1944-7884 | |
| Embargo date | 2030-01-01 | |
