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SECRETORY LEUKOCYTE PROTEASE INHIBITOR EXPRESSION AND HIGH-RISK HPV INFECTION IN ANAL LESIONS OF HIV-POSITIVE PATIENTS
Nicol, Alcina F. et al. | Date Issued: 2016
Author
Nicol, Alcina F.
Brunette, Laurie L.
Nuovo, Gerard J.
Grinsztejn, Beatriz
Friedman, Ruth K.
Veloso, Valdiléa G.
Cunha, Cynthia B.
Coutinho, José R.
Andrade, Cecilia Vianna de
Oliveira, Nathalia S.
Woodham, Andrew W.
Silva, Diana M. da
Kast1, W. Martin
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.
University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA.
Ohio State University. Comprehensive Cancer Center. Columbus, OH, USA.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Patologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.
University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA.
University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA.
University of Southern California. Norris Comprehensive Cancer Center. Department of Molecular Microbiology and Immunology. Department of Obstetrics and Gynecology. Los Angeles, CA, USA.
Abstract
Objective: The aim of this study was to evaluate secretory leukocyte protease inhibitor (SLPI) expression in anal biopsies from HIVpositive (HIV+) individuals, and compare that to anal intraepithelial neoplasia (AIN) diagnoses and human papillomavirus (HPV) status. Design: This is a cross-sectional study of a cohort of 54 HIV+ (31 males and 23 females) from an AIDS clinic in Rio de Janeiro, Brazil. Methods: The study material consisted of anorectal tissue biopsies obtained from HIV+ subjects, which were used to construct tissue microarray paraffin blocks for immunohistochemical analysis of SLPI expression. Biopsies were evaluated by an expert pathologist and classified as low-grade AIN1, high-grade AIN2/3, or normal squamous epithelium. In addition, DNA from the biopsies was extracted and analyzed for the presence of low- or high-risk HPV DNA. Results: Histologically, normal squamous epithelium from the anorectal region showed strong positive SLPI staining in 17/20 (85%) samples. In comparison, 9/17 (53%) dysplastic squamous epithelial samples from AIN1 patients showed strong SLPI staining, and only 5/17 (29%) samples from AIN2/3 patients exhibited strong SPLI staining, which both were significantly fewer than those from normal tissue (P = 0.005). Furthermore, there was a significantly higher proportion of samples in which oncogenic high-risk HPV genotypes were detected in low SLPI-expressing tissues than that in tissues with high SLPI expression (P = 0.040). Conclusions: Taken together these results suggest that low SLPI expression is associated with high-risk HPV infections in the development of AIN.
Keywords in Portuguese
HIV
Inibidor Secretado de Peptidases Leucocitárias
Papillomaviridae
Neoplasias
 
Keywords
secretory leukocyte protease inhibitor,
anal intraepithelial neoplasia
human immunodeficiency virus
human papillomavirus
 
Publisher
Lippincott, Williams & Wilkins
Citation
NICOL, Alcina F. et al. Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients. J Acquir Immune Defic Syndr., v.73, n.1, p.27–33, Sept. 2016.
ISSN
1525-4135