| Author | Corrêa, Jessica Badolato | |
| Author | Sánchez-Arcila, Juan Camilo | |
| Author | Souza, Thiara Manuele Alves de | |
| Author | Barbosa, Luciana Santos | |
| Author | Nunes, Priscila Conrado Guerra | |
| Author | Lima, Monique da Rocha Queiroz | |
| Author | Gandini, Mariana | |
| Author | Filippis, Ana Maria Bispo de | |
| Author | Cunha, Rivaldo Venâncio da | |
| Author | Azeredo, Elzinandes Leal de | |
| Author | Pinto, Luzia Maria de Oliveira | |
| Access date | 2018-01-16T13:13:40Z | |
| Available date | 2018-01-16T13:13:40Z | |
| Document date | 2017 | |
| Citation | CORRÊA, Jessica Badolato; et al. Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection. Immunity, Inflammation and Disease, 13p, 2018 | pt_BR |
| ISSN | 2050-4527 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23923 | |
| Language | eng | pt_BR |
| Publisher | Wiley Open Access | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Zika virus | pt_BR |
| Subject in Portuguese | Linfócitos T | pt_BR |
| Title | Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection | pt_BR |
| Type | Article | |
| DOI | 10.1002/iid3.203 | |
| Abstract | Introduction: Zika virus (ZIKV) and dengue virus (DENV) co-circulated during
latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive
immune responses to these viruses. So far, little is known about human T cell
responses to ZIKV and no reports detail adaptive immune responses during
DENV/ZIKV coinfection.
Methods: Here, we studied T cells responses in well-characterized groups of
DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy
donors. We evaluated chemokine receptors expression and single/multifunctional
frequencies of IFNg, TNF, and IL2-producing T cells during these infections. Even
without antigenic stimulation, it was possible to detect chemokine receptors and
IFNg, TNF, and IL2-producing T cells from all individuals by flow cytometry.
Additionally, PBMCs’ IFNg response to DENV NS1 protein and to polyclonal
stimuli was evaluated by ELISPOT.
Results: DENV and ZIKV infections and DENV/ZIKV coinfections similarly
induced expression of CCR5, CX3CR1, and CXCR3 on CD4 and CD8 T cells.
DENV/ZIKV coinfection decreased the ability of CD4
þ
T cells to produce IFNg
þ
,
TNF
þ
, TNF
þ
IFNg
þ
, and TNF
þ
IL2
þ
, compared to DENV and ZIKV infections. A
higher magnitude of IFNg response to DENV NS1 was found in donors with a
history of dengue infection, however, a hyporesponsiveness was found in acute
DENV, ZIKV, or DENV/ZIKV infected patients, even previously infected with
DENV.
Conclusion: Therefore, we emphasize the potential impact of coinfection on the
immune response from human hosts, mainly in areas where DENV and ZIKV
cocirculate. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Pediatria e Puericultura Martagão Gesteira. Laboratório de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Dengue | pt_BR |
| Subject | Zika virus | pt_BR |
| Subject | T lymphocytes | pt_BR |
