Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23923
Title: Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection
Authors: Corrêa, Jessica Badolato
Sánchez-Arcila, Juan Camilo
Souza, Thiara Manuele Alves de
Barbosa, Luciana Santos
Nunes, Priscila Conrado Guerra
Lima, Monique da Rocha Queiroz
Gandini, Mariana
Filippis, Ana Maria Bispo de
Cunha, Rivaldo Venâncio da
Azeredo, Elzinandes Leal de
Pinto, Luzia Maria de Oliveira
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Pediatria e Puericultura Martagão Gesteira. Laboratório de Genética. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz. Campo Grande, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ. Brasil.
Abstract: Introduction: Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses to ZIKV and no reports detail adaptive immune responses during DENV/ZIKV coinfection. Methods: Here, we studied T cells responses in well-characterized groups of DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy donors. We evaluated chemokine receptors expression and single/multifunctional frequencies of IFNg, TNF, and IL2-producing T cells during these infections. Even without antigenic stimulation, it was possible to detect chemokine receptors and IFNg, TNF, and IL2-producing T cells from all individuals by flow cytometry. Additionally, PBMCs’ IFNg response to DENV NS1 protein and to polyclonal stimuli was evaluated by ELISPOT. Results: DENV and ZIKV infections and DENV/ZIKV coinfections similarly induced expression of CCR5, CX3CR1, and CXCR3 on CD4 and CD8 T cells. DENV/ZIKV coinfection decreased the ability of CD4 þ T cells to produce IFNg þ , TNF þ , TNF þ IFNg þ , and TNF þ IL2 þ , compared to DENV and ZIKV infections. A higher magnitude of IFNg response to DENV NS1 was found in donors with a history of dengue infection, however, a hyporesponsiveness was found in acute DENV, ZIKV, or DENV/ZIKV infected patients, even previously infected with DENV. Conclusion: Therefore, we emphasize the potential impact of coinfection on the immune response from human hosts, mainly in areas where DENV and ZIKV cocirculate.
Keywords: Dengue
Zika virus
T lymphocytes
keywords: Dengue
Zika virus
Linfócitos T
Issue Date: 2017
Publisher: Wiley Open Access
Citation: CORRÊA, Jessica Badolato; et al. Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection. Immunity, Inflammation and Disease, 13p, Dec. 2017.
DOI: 10.1002/iid3.203
ISSN: 2050-4527
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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