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Title: Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune(®)-vaccinated dogs
Authors: Ribeiro, R. A. N.
Teixeira Neto, Rafael Gonçalves
Belo, Vinicius Silva
Ferreira, Eduardo de Castro
Schallig, H. D. F. H.
Silva, Eduardo Sergio da
Affilliation: Universidade Federal de São João del Rei. Divinópolis, MG, Brazil.
Universidade Federal de São João del Rei. Divinópolis, MG, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brazil / Universidade Federal de Juiz de Fora. Governador Valadares, MG, Brazil.
Fundação Oswaldo Cruz Mato Grosso do Sul. Campo Grande, MS, Brazil.
Koninklijk Instituut voor de Tropen. Royal Tropical Institute. KIT Biomedical Research. Parasitology UnitAmsterdamThe Netherlands.
Universidade Federal de São João del Rei. Divinópolis, MG, Brazil.
Abstract: Visceral leishmaniasis (VL) is a serious chronic disease with a lethality rate of up to 10% in humans. In urban areas of Brazil, dogs are the main reservoirs of the etiological agent (Leishmania infantum) of VL, and the Brazilian Ministry of Health recommends the euthanasia of animals that are seropositive in both the immunochromatographic dual path platform rapid test (DPP(®); Bio-Manguinhos) and the enzyme-linked immunosorbent assay (ELISA) with an L. major-like antigen (Bio-Manguinhos). Vaccination is an additional tool in the control of canine VL, but the use of Leishmune(®) (Zoetis Indústria de Produtos Veterinários, São Paulo, SP, Brazil), which contains the fucose mannose ligand (FML) isolated from L. donovani, is not currently recommended by the Brazilian Ministry of Health because vaccinated animals may exhibit positive serology and there are reservations regarding the efficacy of the vaccine. The aims of the present study were: (i) to verify the abilities of the fast agglutination screening test (FAST), the direct agglutination test (DAT), the indirect fluorescent-antibody test (IFAT), the DPP rapid test, and ELISA tests with L. major-like and FML antigens to differentiate between L. infantum-infected and Leishmune(®)-vaccinated dogs, and (ii) to analyze the sensitivities and specificities of the different methods. The reactivities to these tests of Leishmune(®)-vaccinated dogs (n = 71), asymptomatic (n = 20) and symptomatic (n = 20) naturally infected dogs, and unvaccinated healthy control dogs (n = 5) were compared. None of the Leishmune(®)-vaccinated dogs tested seropositive in FAST and DAT, although one dog was reactive to DPP and four dogs to ELISA/L. major-like and IFAT tests. While 69 (97%) of vaccinated dogs reacted to ELISA/FML, only one was seropositive in both ELISA/L. major-like and IFAT tests. Individually, all immunodiagnostic tests presented high specificities and positive likelihood ratios (LR+), and high specificity values were obtained when the tests were considered in pairs. However, sensitivity and LR- values were low for ELISA/L. major-like and IFAT tests individually, and for all pair combinations of tests except for FAST with DPP.
Keywords: Agglutination Tests
Antibodies Protozoan
Antigens Protozoan
Diagnostic Techniques and Procedures
Dog Diseases
keywords: Doenças caninas
Leishmaniose visceral
Issue Date: 2015
Publisher: Springer
Citation: RIBEIRO, R. A. N. et al. Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune(®)-vaccinated dogs. Vet Res Commun., 2015 v. 39, n. 2, p. 87-95.
DOI: 10.1007/s11259-015-9625-6
ISSN: 0165-7380
Copyright: restricted access
Appears in Collections:MS - Artigos de Periódicos
ENSP - Artigos de Periódicos
MG - IRR - Artigos de Periódicos

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