Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24744
Title: 10-valent pneumococcal conjugate vaccine (PCV10) decreases metabolic activity but not nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae
Authors: Andrade, Dafne Carvalho
Borges, Igor Carmo
Bouzas, Maiara Lanna Souza Bacelar
Oliveira, Juliana R
Fukutani, Kiyoshi Ferreira
Queiroz, Artur Trancoso Lopo de
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Van Weyenbergh, Johan Jozef Rosa Maria
Carvalho, Cristiana Maria Costa Nascimento de
Affilliation: Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institutos Nacionais de Ciencia e Tecnologia. São Paulo, SP, Brasil
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institutos Nacionais de Ciencia e Tecnologia. São Paulo, SP, Brasil
Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium
Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Federal University of Bahia School of Medicine. Department of Pediatrics. Salvador, BA, Brazil
Abstract: The effect of pneumococcal vaccination is widely variable when measured by nasopharyngeal carriage of vaccine and non-vaccine targets. The aim of this study was to compare the carriage rates and metabolic activity of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae and Moraxella catarrhalis among children who were or were not vaccinated with PCV10. Methods: We included children with acute respiratory infection aged 6–23 months from a cross-sectional study (CHIADO-IVAS). Nasopharyngeal aspirates were collected and respiratory pathogens were quantified by nCounter digital transcriptomics (Nanostring) and metagenomic sequencing of 16S ribosomal RNA (Illumina). The metabolic rate was calculated by the ratio between RNA transcripts and 16S DNA reads. Results: Out of the 80 patients in this study, 53 were vaccinated with PCV10 and 27 were unvaccinated. There was no difference in nasopharyngeal carriage rates of S. pneumoniae, S. aureus, H. influenzae or M. catarrhalis by either transcriptomic analysis or 16S metagenomics. However, unvaccinated children presented a higher metabolic rate for S. pneumoniae compared to PCV10-vaccinated children (Median [25–75th percentiles]: 126 [22.75–218.41] vs. 0[0–47.83], p = 0.004). Furthermore, unvaccinated children presented a positive correlation between mRNA counts and 16S DNA reads for S. pneumoniae (r = 0.707; p < 0.001) and H. influenzae (r = 0.525; p = 0.005), in contrast to vaccinated children. No such effect was observed for S. aureus and M. catarrhalis. Conclusions: Vaccination by PCV10 exerts a pathogen-specific effect on pneumococcal metabolic rate. Pathogen RNA/DNA ratio might represent a more sensitive readout for vaccine follow-up, as compared to nasopharyngeal carriage
Keywords: Capsular polysaccharides
Metabolism
Nasopharyngeal colonization
Protein D
keywords: Polissacarídeos capsulares
Metabolismo
Colonização nasofaríngea
Proteína D
Issue Date: 2017
Publisher: Elsevier
Citation: ANDRADE, D. C. et al. 10-valent pneumococcal conjugate vaccine (PCV10) decreases metabolic activity but not nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae. Vaccine, 2017.
DOI: 10.1016/j.vaccine.2017.06.048
ISSN: 0264-410X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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