Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24802
Title: Dengue Virus Induces NK Cell Activation through TRAIL Expression during Infection
Authors: Gandini, Mariana
Paiva, Fabienne Petitinga
Marinho, Cíntia Ferreira
Correa, Gladys
Pinto, Luzia Maria de Oliveira
Souza, Luiz José de
Cunha, Rivaldo Venâncio
Kubelka, Claire Fernandes
Azeredo, Elzinandes Leal de
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Plantadores de Cana Hospital. Campos dos Goytacazes, RJ, Brasil.
Universidade Federal do Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande. MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Abstract: Dengue is an acute febrile illness with a wide spectrum of signs and symptoms ranging from mild to severe forms characterized by plasma leakage that can be fatal. NK cells are one of the main effectors in early infection and may play an important role in dengue pathogenesis. We investigated NK cell involvement during dengue infection. A higher frequency of NK cell subsets and TRAIL+NK cells was found in mild DF cases when compared to that in severe cases or healthy donors. NK activation markers such as CD107a and TLR3 were upregulated in patients' cells compared to those in healthy donors. In addition, IL12 related to NK cell activation were upregulated in mild DF cases. In vitro PBMC culture models show that DENV-stimulated and IFNα-stimulated NK cells were able to express TRAIL, suggesting an indirect activation of cells, regarding TRAIL expression. Type I IFN receptor blockage on DENV-stimulated PBMCs showed TRAIL expression on NK cells is partially IFNα dependent. In addition, during PBMC stimulation, TRAIL expression on NK cells was inversely correlated with DENV-positive monocytes. Therefore, we observed DENV-induced activation of NK cell populations. A higher activation of NK cells would promote limited viral spread, resulting in decreased inflammatory response, contributing to protection against dengue severity.
Keywords: Dengue virus
NK Cell
TRAIL expression
Infection
keywords: Vírus da Dengue
Células Matadoras Naturais
Infecção
Issue Date: 2017
Publisher: Hindawi Publishing Corporation
Citation: GANDINI, Mariana; et al. Dengue Virus Induces NK Cell Activation through TRAIL Expression during Infection. Mediators of Inflammation, Volume 2017, Article ID 5649214, 10p, Sept. 2017.
DOI: 10.1155/2017/5649214
ISSN: 0962-9351
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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