Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24850
Title: In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
Authors: Araújo, Sandra Alves
Soares, Alexandra Martins dos Santos
Silva, Carolina Rocha
Almeida Júnior, Eduardo Bezerra
Rocha, Cláudia Quintino
Ferreira, André Teixeira da Silva
Perales, Jonas
Costa Júnior, Livio M.
Affilliation: Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitos. São Luis, MA, Brasil
Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Departamento de Química. Laboratório de Estudos Avançados em Fitomedicina. São Luís, MA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitos. São Luis, MA, Brasil
Abstract: Gastrointestinal nematodes are a significant concern for animal health and well-being, and anthelmintic treatment is mainly performed through the use of chemical products. However, bioactive compounds produced by plants have shown promise for development as novel anthelmintics. The aim of this study is to assess the anthelmintic activity of protein fractions from Spigelia anthelmia on the gastrointestinal nematode Haemonchus contortus. Plant parts were separated into leaves, stems and roots, washed with distilled water, freeze-dried and ground into a fine powder. Protein extraction was performed with sodium phosphate buffer (75 mM, pH 7.0). The extract was fractionated using ammonium sulfate (0-90%) and extensively dialyzed. The resulting fractions were named LPF (leaf protein fraction), SPF (stem protein fraction) and RPF (root protein fraction), and the protein contents and activities of the fractions were analyzed. H. contortus egg hatching (EHA), larval exsheathment inhibition (LEIA) and larval migration inhibition (LMIA) assays were performed. Proteomic analysis was conducted, and high-performance liquid chromatography (HPLC) chromatographic profiles of the fractions were established to identify proteins and possible secondary metabolites. S. anthelmia fractions inhibited H. contortus egg hatching, with LPF having the most potent effects (EC50 0.17 mg mL-1). During LEIA, SPF presented greater efficiency than the other fractions (EC50 0.25 mg mL-1). According to LMIA, the fractions from roots, stems and leaves also reduced the number of larvae, with EC50 values of 0.11, 0.14 and 0.21 mg mL-1, respectively. Protein analysis indicated the presence of plant defense proteins in the S. anthelmia fractions, including protease, protease inhibitor, chitinase and others. Conversely, secondary metabolites were absent in the S. anthemia fractions. These results suggest that S. anthelmia proteins are promising for the control of the gastrointestinal nematode H. contortus.
Keywords: Spigelia anthelmia protein
Haemonchus contortus
anthelmintic effects
In vitro
keywords: efeitos anti-helmínticos
Spigelia anthelmia
Haemonchus contortus
Issue Date: 2017
Publisher: Public Library of Science
Citation: ARAÚJO, Sandra Alves; et al. In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus. PLos One, v.12, n.12, e0189803, Dec. 2017.
DOI: 10.1371/journal.pone.0189803
ISSN: 1932-6203
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
jonas_perales_etal_IOC_2017.pdf1.34 MBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.