| Author | Aquino, Carolina Lessa | |
| Author | Lindow, Janet C | |
| Author | Randall, Arlo | |
| Author | Wunder Junior, Elsio Augusto | |
| Author | Pablo, Jozelyn | |
| Author | Nakajima, Rie | |
| Author | Jasinskas, Algis | |
| Author | Cruz, Jaqueline Silva | |
| Author | Damião, Alcineia Oliveira | |
| Author | Nery Junior, Nívison Ruy Rocha | |
| Author | Ribeiro, Guilherme de Sousa | |
| Author | Costa, Federico | |
| Author | Hagan, José E | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Ko, Albert Icksang | |
| Author | Medeiros, Marco Alberto | |
| Author | Felgner, Philip L | |
| Access date | 2018-02-22T17:09:43Z | |
| Available date | 2018-02-22T17:09:43Z | |
| Document date | 2017 | |
| Citation | AQUINO, C. L. et al. Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis. PLoS Neglected Tropical Diseases , v. 11, n. 1, p. e0005349, 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24919 | |
| Sponsorship | NIH grant U01AI088752 (AIK). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leptospirose | pt_BR |
| Subject in Portuguese | Leptospira Interrogans | pt_BR |
| Subject in Portuguese | Proteome | pt_BR |
| Subject in Portuguese | Anticorpos, Bacteriana | pt_BR |
| Subject in Portuguese | Adulto | pt_BR |
| Subject in Portuguese | Adolescente | pt_BR |
| Title | Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0005349 | |
| Abstract | Leptospirosis is an important zoonotic disease worldwide. Humans usually present a mild non-specific febrile illness, but a proportion of them develop more severe outcomes, such as multi-organ failure, lung hemorrhage and death. Such complications are thought to depend on several factors, including the host immunity. Protective immunity is associated with humoral immune response, but little is known about the immune response mounted during naturally-acquired Leptospira infection. Here, we used protein microarray chip to profile the antibody responses of patients with
severe and mild leptospirosis against the complete Leptospira interrogans serovar Copenhageni
predicted ORFeome. We discovered a limited number of immunodominant antigens,
with 36 antigens specific to patients, of which 11 were potential serodiagnostic antigens,
identified at acute phase, and 33 were potential subunit vaccine targets, detected after
recovery. Moreover, we found distinct antibody profiles in patients with different clinical outcomes:
in the severe group, overall IgM responses do not change and IgG responses
increase over time, while both IgM and IgG responses remain stable in the mild patient
group. Analyses of individual patients' responses showed that >74% of patients in the
severe group had significant IgG increases over time compared to 29% of patients in the
mild group. Additionally, 90% of IgM responses did not change over time in the mild group,
compared to ~51% in the severe group. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA | pt_BR |
| Affilliation | Antigen Discovery Inc. Irvine, CA, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA | pt_BR |
| Affilliation | University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA | pt_BR |
| Affilliation | University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA | pt_BR |
| Affilliation | University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil | pt_BR |
| Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA | pt_BR |
| Subject | Leptospirosis | pt_BR |
| Subject | Leptospira interrogans | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Antibodies, Bacterial | pt_BR |
| Subject | Adult | pt_BR |
| Subject | Adolescent | pt_BR |
