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https://www.arca.fiocruz.br/handle/icict/24919
DISTINCT ANTIBODY RESPONSES OF PATIENTS WITH MILD AND SEVERE LEPTOSPIROSIS DETERMINED BY WHOLE PROTEOME MICROARRAY ANALYSIS
Leptospira Interrogans
Proteome
Anticorpos, Bacteriana
Adulto
Adolescente
Author
Aquino, Carolina Lessa
Lindow, Janet C
Randall, Arlo
Wunder Junior, Elsio Augusto
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Cruz, Jaqueline Silva
Damião, Alcineia Oliveira
Nery Junior, Nívison Ruy Rocha
Ribeiro, Guilherme de Sousa
Costa, Federico
Hagan, José E
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
Lindow, Janet C
Randall, Arlo
Wunder Junior, Elsio Augusto
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Cruz, Jaqueline Silva
Damião, Alcineia Oliveira
Nery Junior, Nívison Ruy Rocha
Ribeiro, Guilherme de Sousa
Costa, Federico
Hagan, José E
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
Affilliation
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Antigen Discovery Inc. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Antigen Discovery Inc. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Abstract
Leptospirosis is an important zoonotic disease worldwide. Humans usually present a mild non-specific febrile illness, but a proportion of them develop more severe outcomes, such as multi-organ failure, lung hemorrhage and death. Such complications are thought to depend on several factors, including the host immunity. Protective immunity is associated with humoral immune response, but little is known about the immune response mounted during naturally-acquired Leptospira infection. Here, we used protein microarray chip to profile the antibody responses of patients with
severe and mild leptospirosis against the complete Leptospira interrogans serovar Copenhageni
predicted ORFeome. We discovered a limited number of immunodominant antigens,
with 36 antigens specific to patients, of which 11 were potential serodiagnostic antigens,
identified at acute phase, and 33 were potential subunit vaccine targets, detected after
recovery. Moreover, we found distinct antibody profiles in patients with different clinical outcomes:
in the severe group, overall IgM responses do not change and IgG responses
increase over time, while both IgM and IgG responses remain stable in the mild patient
group. Analyses of individual patients' responses showed that >74% of patients in the
severe group had significant IgG increases over time compared to 29% of patients in the
mild group. Additionally, 90% of IgM responses did not change over time in the mild group,
compared to ~51% in the severe group.
Keywords in Portuguese
LeptospiroseLeptospira Interrogans
Proteome
Anticorpos, Bacteriana
Adulto
Adolescente
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