Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24919
Title: Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis
Authors: Aquino, Carolina Lessa
Lindow, Janet C
Randall, Arlo
Wunder Junior, Elsio Augusto
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Cruz, Jaqueline Silva
Damião, Alcineia Oliveira
Nery Junior, Nívison Ruy Rocha
Ribeiro, Guilherme de Sousa
Costa, Federico
Hagan, José E
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
Affilliation: Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Antigen Discovery Inc. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, Brazil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USA
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USA
Abstract: Leptospirosis is an important zoonotic disease worldwide. Humans usually present a mild non-specific febrile illness, but a proportion of them develop more severe outcomes, such as multi-organ failure, lung hemorrhage and death. Such complications are thought to depend on several factors, including the host immunity. Protective immunity is associated with humoral immune response, but little is known about the immune response mounted during naturally-acquired Leptospira infection. Here, we used protein microarray chip to profile the antibody responses of patients with severe and mild leptospirosis against the complete Leptospira interrogans serovar Copenhageni predicted ORFeome. We discovered a limited number of immunodominant antigens, with 36 antigens specific to patients, of which 11 were potential serodiagnostic antigens, identified at acute phase, and 33 were potential subunit vaccine targets, detected after recovery. Moreover, we found distinct antibody profiles in patients with different clinical outcomes: in the severe group, overall IgM responses do not change and IgG responses increase over time, while both IgM and IgG responses remain stable in the mild patient group. Analyses of individual patients' responses showed that >74% of patients in the severe group had significant IgG increases over time compared to 29% of patients in the mild group. Additionally, 90% of IgM responses did not change over time in the mild group, compared to ~51% in the severe group.
Keywords: Leptospirosis
Leptospira interrogans
Proteome
Antibodies, Bacterial
Adult
Adolescent
keywords: Leptospirose
Leptospira Interrogans
Proteome
Anticorpos, Bacteriana
Adulto
Adolescente
Issue Date: 2017
Publisher: Public Library of Science
Citation: AQUINO, C. L. et al. Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis. PLoS Neglected Tropical Diseases , v. 11, n. 1, p. e0005349, 2017.
DOI: 10.1371/journal.pntd.0005349
ISSN: 1935-2727
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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