Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24926
Title: Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis
Authors: Gardinassi, Luiz Gustavo Araujo
Vieira, Thiago Soares de Souza
Silva, Naila O. da
Garcia, Gustavo Rocha
Borges, Valéria de Matos
Campos, Roseane Nunes de Santana
Almeida, Roque Pacheco de
Santos, Isabel Kiney Ferreira de Miranda
Saraiva, Elvira Maria
Affilliation: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, Brazil
Universidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, Brazil
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil
Abstract: Infections with parasites of the Leishmania donovani complex result in clinical outcomes that range from asymptomatic infection to severe and fatal visceral leishmaniasis (VL). Neutrophils are major players of the immune response against Leishmania, but their contribution to distinct states of infection is unknown. Gene expression data suggest the activation of the NETosis pathway during human visceral leishmaniasis. Thus, we conducted an exploratory study to evaluate NET-related molecules in retrospective sera from VL patients, asymptomatic individuals and uninfected endemic controls. Results: We demonstrate that VL patients and asymptomatic individuals exhibit differential regulation of molecules associated with neutrophil extracellular traps (NET). These differences were observed at the transcriptional level of genes encoding NET-associated proteins; in quantifications of cell free DNA and metalloproteinase 9; and in enzymatic activity of DNAse and elastase. Moreover, multivariate analysis resulted in class-specific signatures, and ROC curves demonstrate the ability of these molecules in discriminating asymptomatic infection from uninfected controls. Conclusion: Molecules that are associated with NETs are differentially regulated between distinct states of infection with L. infantum, suggesting that NETs might have distinct roles depending on the clinical status of infection. Although unlikely to be exclusive for VL, these signatures can be useful to better characterize asymptomatic infections in endemic regions of this disease.
Keywords: Neutrophil extracellular traps
NETosis
Leishmania infantum
Visceral leishmaniasis
Asymptomatic infection
keywords: Armadilhas extracelulares de Neutrófilos
NETosis
Leishmania infantum
Leishmaniose visceral
Infecção assintomática
Issue Date: 2017
Publisher: BioMed Central
Citation: GARDINASSI, L. G. A. et al.Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. Parasites & Vectors, v. 10, p. 285, 2017.
DOI: 10.1186/s13071-017-2222-5
ISSN: 1756-3305
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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