Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24950
Title: In silico analysis of amino acid variation in human respiratory Syncytial virus: insights into immunodiagnostics
Authors: Souza, Claudemir
Zanchin, Nilson Ivo Tonin
Krieger, Marco Aurélio
Ludwig, Adriana
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Abstract: The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes.This paper has the objectiv of to analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. It found that the mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. How main conclusions, we have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
Keywords: Respiratory Syncytial Virus
Human
Genetic Variation
Antigens
Keywords in spanish: Virus Sincitial Respiratorio Humano
Variación Genética
Antígenos
keywords: Diagnóstico
DeCS: Vírus Sincicial Respiratório Humano
Variação Genética
Antígenos
Issue Date: 2017
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: SOUZA, Claudemir et al. In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 112, n.10, p. 655-663, 2017.
DOI: 10.1590/0074-02760170013
ISSN: 1678-8060
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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