Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25280
Title: Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil
Authors: Balassiano, Ilana T.
Filho, Joaquim dos Santos
Brazil, Juliana M. Vital
Nouér, Simone A.
Souza, Claudia R. C.
Brazier, Jon S.
Ferreira, Eliane de O.
Domingues, Regina M. C. P.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Biologia de Anaeróbios.Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Biologia de Anaeróbios.Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Escola de Medicina. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Coordenação de Controle de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Coordenação de Controle de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.
University Hospital of Wales. Anaerobe Reference Unit. Cardiff, United Kingdom.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Biologia de Anaeróbios.Rio de Janeiro, RJ, Brasil.
Abstract: Clostridium difficile is an important nosocomial enteric pathogen and is the etiological agent of pseudomembranous colites. Recently, the rates of C. difficile infection (CDI) have increased worldwide, but in Brazil few data about this situation and the incidence of clonal types of C. difficile exist. This study aimed to isolate and characterize C. difficile strains from samples obtained of a university hospital (HUCFF) in Rio de Janeiro city, Brazil. CDI was identified by ELISA in 27.1% of HUCFF-in-patients enrolled in the study, and the bacterium was recovered from eight of these fecal samples. All strains, except one, presented tcdA and tcdB genes and presented neither the cdtA and cdtB genes nor any significant deletions in the tcdC gene. All strains were sensitive to metronidazole, vancomycin and moxifloxacin, and resistant to clindamycin, ciprofloxacin and levofloxacin. PCR-ribotyping and PFGE revealed four different clonal types among the isolates. The Brazilian PCR-ribotype 133 accounted for 50% of strains isolated, and PCR-ribotype 233 strains were obtained from 25% of the in-patients. The prevalence and resurgence of the Brazilian PCR-ribotype 133 among the hospitalized patients of HUCFF was established, and cross-infection of different patients associated to the same PCR-ribotypes was detected. Our results emphasize the importance of the diagnosis and control of CDI in order to prevent the emergence of specific clones that can lead to C. difficile-associated outbreaks in Brazilian hospitals.
Keywords: Clostridium difficile
Clostridium difficile infection
PCR-ribotypes
Antimicrobial resistance
keywords: Clostridium difficile
Antimicrobial resistance
Infecção
Ribotipagem
Reação em Cadeia da Polimerase
Rio de Janeiro
Issue Date: 2011
Publisher: 1572-9699
Citation: BALASSIANO, Ilana T. et al. Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil. Antonie van Leeuwenhoek, v. 99, 249–255, 2011.
DOI: 10.1007/s10482-010-9483-8
ISSN: 0003-6072
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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