Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25377
Title: Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
Authors: Santos, Andréa Gonçalves dos
Mendes, Érica Araujo
Oliveira, Rafael P. de
Faria, Ana Maria Caetano de
Sousa, Aurizangela Oliveira de
Pirovani, Carlos Priminho
Araújo, Fernanda Fortes de
Carvalho, Andréa Teixeira de
Costa, Marliete Carvalho
Santos, Daniel Assis
Montoya, Quimi V.
Rodrigues, Andre
Santos, Jane L. dos
Affilliation: Universidade Estadual de Santa Cruz. Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos. Ilhéus, BA, Brazil
Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Microbiologia. São Paulo, Brazil
Instituto Federal do Paraná. Palmas, TO, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, MG, Brazil
Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pós-Graduação em Sanidade e Produção Animal nos Trópicos. Uberaba, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Estadual de São Paulo. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brazil
Universidade Estadual de São Paulo. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brazil
Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Microbiologia. São Paulo, Brazil
Abstract: The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis.
Keywords: biocontrol agent
phagocytosis
conidia
PRRs
Candida parapsilosis
keywords: agentes de controle biologico
fagocitose
conidia
PRRs
Candida parapsilosis
Issue Date: 2017
Publisher: Frontiers Research Foundation
Citation: SANTOS, Andréa Gonçalves dos et al. Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization. Front Microbiol., v. 8, p. 1681, 2017
DOI: 10.3389/fmicb.2017.01681
ISSN: 1664-302X
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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