| Author | Chotiwan, Nunya | |
| Author | Brewster, Connie D. | |
| Author | Magalhaes, Tereza | |
| Author | Weger-Lucarelli, James | |
| Author | Duggal, Nisha K. | |
| Author | Rückert, Claudia | |
| Author | Nguyen, Chilinh | |
| Author | Luna, Selene M. Garcia | |
| Author | Fauver, Joseph R. | |
| Author | Andre, Barb | |
| Author | Gray, Meg | |
| Author | Black, William C. | |
| Author | Kading, Rebekah C. | |
| Author | Ebel, Gregory D. | |
| Author | Kuan, Guillermina | |
| Author | Balmaseda, Angel | |
| Author | Jaenisch, Thomas | |
| Author | Marques, Ernesto T. A. | |
| Author | Brault, Aaron C. | |
| Author | Harris, Eva | |
| Author | Foy, Brian D. | |
| Author | Quackenbush, Sandra L. | |
| Author | Perera, Rushika | |
| Author | Rovnak, Joel | |
| Access date | 2018-03-22T12:58:01Z | |
| Available date | 2018-03-22T12:58:01Z | |
| Document date | 2017 | |
| Citation | CHOTIWAN, N. et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Science Translational Medicine, v. 9, n. 388, p. eaag0538, 3 maio 2017. | pt_BR |
| ISSN | 1946-6242 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25454 | |
| Sponsorship | A pesquisa relatada nesta publicação foi apoiada pelo Instituto Nacional de Alergia e Doenças Infecciosas (NIAID) do NIH (prêmios nos. RO1AI067380 para JW-L., CR, JRF e GDE; R01AI0833680 para WCB; R21AI129464 para BDF , CN, MG, TM e ETAM; R01AI099631 para AB; e R21AI129488-01 para RP e JR) e o Programa Internacional de Colaboração em Doenças Infecciosas do NIH / NIAID (U01AI088647). O conteúdo é de exclusiva responsabilidade dos autores e não representa necessariamente as opiniões oficiais do NIH. Apoio adicional foi dado pela Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (APQ-0302-4.01 / 13 ao ETAM) e pela União Européia (FP7-281803 IDAMS) ( 41). O SMGL é apoiado pelo Fogarty Training Grant 2D43TW001130-08 “Treinamento em prevenção e controle da dengue”. O NKD e o ACB receberam apoio dos Centros de Controle e Prevenção de Doenças. Esta submissão representa os pontos de vista exclusivamente dos autores e não constitui os dos Centros de Controle e Prevenção de Doenças ou o governo dos EUA. BA, CDB, RP, RCK, SLQ e JR foram apoiados pelo Departamento de Microbiologia, Imunologia e Patologia e pela Faculdade de Medicina Veterinária e Ciências Biomédicas da Colorado State University, e BDF, RP, TM e NC foram apoiados por o Gabinete do Vice-Presidente de Pesquisa da Universidade do Estado do Colorado. | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Vírus Zika | pt_BR |
| Subject in Portuguese | RNA | pt_BR |
| Title | Rapid and specific detection of Asian- and African-lineage Zika viruses | pt_BR |
| Type | Article | |
| DOI | 10.1126/scitranslmed.aag0538 | |
| Abstract | Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Ministry of Health. Centro de Salud Sócrates Flores Vivas. Managua, Nicaragua. | pt_BR |
| Affilliation | Ministry of Health. Centro Nacional de Diagnóstico y Referencia. Laboratorio Nacional de Virología. Managua, Nicaragua. | pt_BR |
| Affilliation | Heidelberg University Hospital. Department for Infectious Diseases. Section Clinical Tropical Medicine. Heidelberg, Germany / German Centre for Infection Research (DZIF). Partner site Heidelberg. Heidelberg, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / University of Pittsburgh. School of Public Health. Center for Vaccine Research. Pittsburgh, USA. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. Fort Collins, CO, USA. | pt_BR |
| Affilliation | University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Subject | Zika virus | pt_BR |
| Subject | RNA | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Culicidae | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Técnicas de Amplificação de Ácido Nucleico / métodos | pt_BR |
| DeCS | RNA Viral | pt_BR |
| DeCS | Vírus Zika | pt_BR |
| Embargo date | 2043-03-22 | |
