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RAPID AND SPECIFIC DETECTION OF ASIAN- AND AFRICAN-LINEAGE ZIKA VIRUSES
Chotiwan, Nunya et al. | Date Issued: 2017
Author
Chotiwan, Nunya
Brewster, Connie D.
Magalhaes, Tereza
Weger-Lucarelli, James
Duggal, Nisha K.
Rückert, Claudia
Nguyen, Chilinh
Luna, Selene M. Garcia
Fauver, Joseph R.
Andre, Barb
Gray, Meg
Black, William C.
Kading, Rebekah C.
Ebel, Gregory D.
Kuan, Guillermina
Balmaseda, Angel
Jaenisch, Thomas
Marques, Ernesto T. A.
Brault, Aaron C.
Harris, Eva
Foy, Brian D.
Quackenbush, Sandra L.
Perera, Rushika
Rovnak, Joel
Affilliation
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Ministry of Health. Centro de Salud Sócrates Flores Vivas. Managua, Nicaragua.
Ministry of Health. Centro Nacional de Diagnóstico y Referencia. Laboratorio Nacional de Virología. Managua, Nicaragua.
Heidelberg University Hospital. Department for Infectious Diseases. Section Clinical Tropical Medicine. Heidelberg, Germany / German Centre for Infection Research (DZIF). Partner site Heidelberg. Heidelberg, Germany.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / University of Pittsburgh. School of Public Health. Center for Vaccine Research. Pittsburgh, USA.
Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. Fort Collins, CO, USA.
University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Arthropod-borne Infectious Disease Laboratories. Fort Collins, CO, USA.
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Abstract
Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.
Keywords in Portuguese
Vírus Zika
RNA
 
Keywords
Zika virus
RNA
 
DeCS
Animais
Culicidae
Humanos
Técnicas de Amplificação de Ácido Nucleico / métodos
RNA Viral
Vírus Zika
 
Citation
CHOTIWAN, N. et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Science Translational Medicine, v. 9, n. 388, p. eaag0538, 3 maio 2017.
DOI
10.1126/scitranslmed.aag0538
ISSN
1946-6242