| Author | Moura, Anna Paula V. | |
| Author | Santos, Luiza C. B. | |
| Author | Brito, Carlos Ramon Nascimento | |
| Author | Valencia, Edward | |
| Author | Giusta, Caroline Junqueira | |
| Author | P. Filho, Adalberto A | |
| Author | Sant’Anna, Mauricio R. V. | |
| Author | Gontijo, Nelder F. | |
| Author | Bartholomeu, Daniella Castanheira | |
| Author | Fujiwara, Ricardo Toshio | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | McKay, Craig S. | |
| Author | Sanhueza, Carlos A. | |
| Author | Finn, M. G. | |
| Author | Marques, Alexandre Ferreira | |
| Access date | 2018-03-23T17:21:02Z | |
| Available date | 2018-03-23T17:21:02Z | |
| Document date | 2017 | |
| Citation | MOURA, Anna Paula V. et al. Virus-like Particle Display of the α-Gal Carbohydrate for Vaccination against Leishmania Infection. ACS Cent Sci., v. 3, n. 9, p.1026-1031, 2017 | pt_BR |
| ISSN | 2374-7943 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25474 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Leishmania amazonensis | pt_BR |
| Subject in Portuguese | vacinaçao | pt_BR |
| Title | Virus-like Particle Display of the α-Gal Carbohydrate for Vaccination against Leishmania Infection | pt_BR |
| Type | Article | |
| DOI | 10.1021/acscentsci.7b00311 | |
| Abstract | Secreted and surface-displayed carbohydrates are essential for virulence and viability of many parasites, including for immune system evasion. We have identified the α-Gal trisaccharide epitope on the surface of the protozoan parasites Leishmania infantum and Leishmania amazonensis, the etiological agents of visceral and cutaneous leishmaniasis, respectively, with the latter bearing larger amounts of α-Gal than the former. A polyvalent α-Gal conjugate on the immunogenic Qβ virus-like particle was tested as a vaccine against Leishmania infection in a C57BL/6 α-galactosyltransferase knockout mouse model, which mimics human hosts in producing high titers of anti-α-Gal antibodies. As expected, α-Gal-T knockout mice infected with promastigotes of both Leishmania species showed significantly lower parasite load in the liver and slightly decreased levels in the spleen, compared with wild-type mice. Vaccination with Qβ–α-Gal nanoparticles protected the knockout mice against Leishmania challenge, eliminating the infection and proliferation of parasites in the liver and spleen as probed by qPCR. The α-Gal epitope may therefore be considered as a vaccine candidate to block human cutaneous and visceral leishmaniasis. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States | pt_BR |
| Affilliation | School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States | pt_BR |
| Affilliation | School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | vaccination | pt_BR |
| Embargo date | 2090-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
