Title:	Virus-like Particle Display of the α-Gal Carbohydrate for Vaccination against Leishmania Infection
Authors:	Moura, Anna Paula V. Santos, Luiza C. B. Brito, Carlos Ramon Nascimento Valencia, Edward Giusta, Caroline Junqueira P. Filho, Adalberto A Sant’Anna, Mauricio R. V. Gontijo, Nelder F. Bartholomeu, Daniella C. Fujiwara, Ricardo Toshio Gazzinelli, Ricardo Tostes McKay, Craig S. Sanhueza, Carlos A. Finn, M. G. Marques, Alexandre Ferreira
Affilliation:	Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States School of Chemistry and Biochemistry. School of Biology. Georgia Institute of Technology. Atlanta, Georgia, United States Universidade Federal de Minas Gerais. Departamento de Parasitologia. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil
Abstract:	Secreted and surface-displayed carbohydrates are essential for virulence and viability of many parasites, including for immune system evasion. We have identified the α-Gal trisaccharide epitope on the surface of the protozoan parasites Leishmania infantum and Leishmania amazonensis, the etiological agents of visceral and cutaneous leishmaniasis, respectively, with the latter bearing larger amounts of α-Gal than the former. A polyvalent α-Gal conjugate on the immunogenic Qβ virus-like particle was tested as a vaccine against Leishmania infection in a C57BL/6 α-galactosyltransferase knockout mouse model, which mimics human hosts in producing high titers of anti-α-Gal antibodies. As expected, α-Gal-T knockout mice infected with promastigotes of both Leishmania species showed significantly lower parasite load in the liver and slightly decreased levels in the spleen, compared with wild-type mice. Vaccination with Qβ–α-Gal nanoparticles protected the knockout mice against Leishmania challenge, eliminating the infection and proliferation of parasites in the liver and spleen as probed by qPCR. The α-Gal epitope may therefore be considered as a vaccine candidate to block human cutaneous and visceral leishmaniasis.
Keywords:	Leishmania infantum Leishmania amazonensis vaccination
keywords:	Leishmania infantum Leishmania amazonensis vacinaçao
Issue Date:	2017
Publisher:	American Chemical Society
Citation:	MOURA, Anna Paula V. et al. Virus-like Particle Display of the α-Gal Carbohydrate for Vaccination against Leishmania Infection. ACS Cent Sci., v. 3, n. 9, p.1026-1031, 2017
DOI:	10.1021/acscentsci.7b00311
ISSN:	2374-7943
Copyright:	restricted access
Appears in Collections:	MG - IRR - Artigos de Periódicos

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