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THE IRON-DEPENDENT MITOCHONDRIAL SUPEROXIDE DISMUTASE SODA PROMOTESLEISHMANIA VIRULENCE
Diferenciação
Ferro superóxido dismutase
Mitocôndria
Sinalização redox
Superóxido dismutase (SOD
Differentiation
Iron superoxide dismutase
Mitochondria
Redox signaling
Superoxide dismutase (SOD)
Author
Affilliation
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
Abstract
Leishmaniasis is one of the leading globally neglected diseases, affecting millions of people worldwide.Leishmaniainfection depends on the ability of insect-transmitted metacyclic promastigotes to invade mammalian hosts, differentiate into amastigotes, and replicate inside macrophages. To counter the hostile oxidative environment inside macrophages, these protozoans contain anti-oxidant systems that include iron-dependent superoxide dismutases (SODs) in mitochondria and glycosomes. Increasing evidence suggests that in addition to this protective role,Leishmaniamitochondrial SOD may also initiate H2O2-mediated redox signaling that regulates gene expression and metabolic changes associated with differentiation into virulent forms. To investigate this hypothesis, we examined the specific role of SODA, the mitochondrial SOD isoform inLeishmania amazonensisOur inability to generateL. amazonensis SODAnull mutants and the lethal phenotype observed following RNAi-mediated silencing of theTrypanosoma brucei SODAortholog suggests that SODA is essential for trypanosomatid survival.L. amazonensismetacyclic promastigotes lacking oneSODAallele failed to replicate in macrophages and were severely attenuated in their ability to generate cutaneous lesions in mice. Reduced expression of SODA also resulted in mitochondrial oxidative damage and failure ofSODA/ΔsodApromastigotes to differentiate into axenic amastigotes. SODA expression above a critical threshold was also required for the development of metacyclic promastigotes, asSODA/ΔsodAcultures were strongly depleted in this infective form and more susceptible to reactive oxygen species (ROS)-induced stress. Collectively, our data suggest that SODA promotesLeishmaniavirulence by protecting the parasites against mitochondrion-generated oxidative stress and by initiating ROS-mediated signaling mechanisms required for the differentiation of infective forms.
Keywords in Portuguese
LeishmaniaDiferenciação
Ferro superóxido dismutase
Mitocôndria
Sinalização redox
Superóxido dismutase (SOD
Keywords
LeishmaniaDifferentiation
Iron superoxide dismutase
Mitochondria
Redox signaling
Superoxide dismutase (SOD)
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