| Author | Oliveira, Andresa Pereira de | |
| Author | Castro, Maria Carolina Accioly Brelaz de | |
| Author | Almeida, Amanda Ferreira de | |
| Author | Souza, Marina de Assis | |
| Author | Oliveira, Beatriz Coutinho de | |
| Author | Reis, Luiza Campos | |
| Author | Goto, Hiro | |
| Author | Brito, Maria Edileuza Felinto de | |
| Author | Celeste, Beatriz Julieta | |
| Author | Martins-Filho, Olindo Assis | |
| Author | Pereira, Valéria Rêgo Alves | |
| Access date | 2018-04-02T13:06:06Z | |
| Available date | 2018-04-02T13:06:06Z | |
| Document date | 2013 | |
| Citation | DE OLIVEIRA, A. P. et al. Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G. Journal of Immunological Methods, v. 387, n. 1–2, p. 245–253, 31 jan. 2013. | pt_BR |
| ISSN | 1872-7905 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25553 | |
| Sponsorship | Este trabalho foi apoiado pela Fundação de Amparo à Pesquisa do Estado de Pernambuco (FACEPE) , pelo Conselho Nacional de Pesquisa (CNPq) e pela Fundação Oswaldo Cruz (FIOCRUZ) . | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Subject in Portuguese | Ensaio de imunofluorescência indireta | pt_BR |
| Subject in Portuguese | IgG | pt_BR |
| Subject in Portuguese | L. ( V. ) braziliensis | pt_BR |
| Subject in Portuguese | Leishmaniose tegumentar americana | pt_BR |
| Title | Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jim.2012.11.002 | |
| Abstract | The aim of this study was to compare the techniques of indirect immunofluorescence assay (IFA) and flow cytometry to clinical and laboratorial evaluation of patients before and after clinical cure and to evaluate the applicability of flow cytometry in post-therapeutic monitoring of patients with American tegumentary leishmaniasis (ATL). Sera from 14 patients before treatment (BT), 13 patients 1 year after treatment (AT), 10 patients 2 and 5 years AT were evaluated. The results from flow cytometry were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). The 1:256 sample dilution allowed us to differentiate individuals BT and AT. Comparative analysis of IFA and flow cytometry by ROC (receiver operating characteristic curve) showed, respectively, AUC (area under curve)=0.8 (95% CI=0.64-0.89) and AUC=0.90 (95% CI=0.75-0.95), demonstrating that the flow cytometry had equivalent accuracy. Our data demonstrated that 20% was the best cut-off point identified by the ROC curve for the flow cytometry assay. This test showed a sensitivity of 86% and specificity of 77% while the IFA had a sensitivity of 78% and specificity of 85%. The after-treatment screening, through comparative analysis of the technique performance indexes, 1, 2 and 5 years AT, showed an equal performance of the flow cytometry compared with the IFA. However, flow cytometry shows to be a better diagnostic alternative when applied to the study of ATL in the cure criterion. The information obtained in this work opens perspectives to monitor cure after treatment of ATL. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Minas Gerais, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Indirect immunofluorescence assay | pt_BR |
| Subject | IgG | pt_BR |
| Subject | L. (V.) braziliensis | pt_BR |
| Subject | American tegumentary leishmaniasis | pt_BR |
| DeCS | Adolescente | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Envelhecido | pt_BR |
| DeCS | Anticorpos, Protozoários / sangue | pt_BR |
| DeCS | Anticorpos, protozoários / imunologia | pt_BR |
| DeCS | Fêmea | pt_BR |
| DeCS | Citometria de Fluxo / métodos | pt_BR |
| DeCS | Técnica de Anticorpo Fluorescente Indireta / métodos * | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Imunoglobulina G / imunologia | pt_BR |
| DeCS | Imunoglobulina G / uso terapêutico | pt_BR |
| DeCS | Leishmania braziliensis / imunologia | pt_BR |
| DeCS | Leishmaniose cutânea / sangue | pt_BR |
| DeCS | Leishmaniose cutânea / quimioterapia | pt_BR |
| DeCS | Leishmaniose cutânea / imunologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Meia idade | pt_BR |
| DeCS | Avaliação de Resultados (Cuidados de Saúde) / métodos | pt_BR |
| DeCS | Reprodutibilidade dos Resultados | pt_BR |
| DeCS | Fatores de Tempo | pt_BR |
| DeCS | Adulto jovem | pt_BR |
| Embargo date | 2050-01-01 | |
