Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25553
Title: Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G
Authors: Oliveira, Andresa Pereira de
Castro, Maria Carolina Accioly Brelaz de
Almeida, Amanda Ferreira de
Souza, Marina de Assis
Oliveira, Beatriz Coutinho de
Reis, Luiza Campos
Goto, Hiro
Brito, Maria Edileuza Felinto de
Celeste, Beatriz Julieta
Martins-Filho, Olindo Assis
Pereira, Valéria Rêgo Alves
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil
Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Minas Gerais, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Abstract: The aim of this study was to compare the techniques of indirect immunofluorescence assay (IFA) and flow cytometry to clinical and laboratorial evaluation of patients before and after clinical cure and to evaluate the applicability of flow cytometry in post-therapeutic monitoring of patients with American tegumentary leishmaniasis (ATL). Sera from 14 patients before treatment (BT), 13 patients 1 year after treatment (AT), 10 patients 2 and 5 years AT were evaluated. The results from flow cytometry were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). The 1:256 sample dilution allowed us to differentiate individuals BT and AT. Comparative analysis of IFA and flow cytometry by ROC (receiver operating characteristic curve) showed, respectively, AUC (area under curve)=0.8 (95% CI=0.64-0.89) and AUC=0.90 (95% CI=0.75-0.95), demonstrating that the flow cytometry had equivalent accuracy. Our data demonstrated that 20% was the best cut-off point identified by the ROC curve for the flow cytometry assay. This test showed a sensitivity of 86% and specificity of 77% while the IFA had a sensitivity of 78% and specificity of 85%. The after-treatment screening, through comparative analysis of the technique performance indexes, 1, 2 and 5 years AT, showed an equal performance of the flow cytometry compared with the IFA. However, flow cytometry shows to be a better diagnostic alternative when applied to the study of ATL in the cure criterion. The information obtained in this work opens perspectives to monitor cure after treatment of ATL.
Keywords: Flow cytometry
Indirect immunofluorescence assay
IgG
L. (V.) braziliensis
American tegumentary leishmaniasis
keywords: Citometria de fluxo
Ensaio de imunofluorescência indireta
IgG
L. ( V. ) braziliensis
Leishmaniose tegumentar americana
DeCS: Adolescente
Adulto
Envelhecido
Anticorpos, Protozoários / sangue
Anticorpos, protozoários / imunologia
Fêmea
Citometria de Fluxo / métodos
Técnica de Anticorpo Fluorescente Indireta / métodos *
Humanos
Imunoglobulina G / imunologia
Imunoglobulina G / uso terapêutico
Leishmania braziliensis / imunologia
Leishmaniose cutânea / sangue
Leishmaniose cutânea / quimioterapia
Leishmaniose cutânea / imunologia
Masculino
Meia idade
Avaliação de Resultados (Cuidados de Saúde) / métodos
Reprodutibilidade dos Resultados
Fatores de Tempo
Adulto jovem
Issue Date: 2013
Citation: DE OLIVEIRA, A. P. et al. Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G. Journal of Immunological Methods, v. 387, n. 1–2, p. 245–253, 31 jan. 2013.
DOI: 10.1016/j.jim.2012.11.002
ISSN: 1872-7905
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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