Title:	Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G
Authors:	Oliveira, Andresa Pereira de Castro, Maria Carolina Accioly Brelaz de Almeida, Amanda Ferreira de Souza, Marina de Assis Oliveira, Beatriz Coutinho de Reis, Luiza Campos Goto, Hiro Brito, Maria Edileuza Felinto de Celeste, Beatriz Julieta Martins-Filho, Olindo Assis Pereira, Valéria Rêgo Alves
Affilliation:	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. Universidade de São Paulo. Faculdade de Medicina e Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Minas Gerais, Brazil. Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Abstract:	The aim of this study was to compare the techniques of indirect immunofluorescence assay (IFA) and flow cytometry to clinical and laboratorial evaluation of patients before and after clinical cure and to evaluate the applicability of flow cytometry in post-therapeutic monitoring of patients with American tegumentary leishmaniasis (ATL). Sera from 14 patients before treatment (BT), 13 patients 1 year after treatment (AT), 10 patients 2 and 5 years AT were evaluated. The results from flow cytometry were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). The 1:256 sample dilution allowed us to differentiate individuals BT and AT. Comparative analysis of IFA and flow cytometry by ROC (receiver operating characteristic curve) showed, respectively, AUC (area under curve)=0.8 (95% CI=0.64-0.89) and AUC=0.90 (95% CI=0.75-0.95), demonstrating that the flow cytometry had equivalent accuracy. Our data demonstrated that 20% was the best cut-off point identified by the ROC curve for the flow cytometry assay. This test showed a sensitivity of 86% and specificity of 77% while the IFA had a sensitivity of 78% and specificity of 85%. The after-treatment screening, through comparative analysis of the technique performance indexes, 1, 2 and 5 years AT, showed an equal performance of the flow cytometry compared with the IFA. However, flow cytometry shows to be a better diagnostic alternative when applied to the study of ATL in the cure criterion. The information obtained in this work opens perspectives to monitor cure after treatment of ATL.
Keywords:	Flow cytometry Indirect immunofluorescence assay IgG L. (V.) braziliensis American tegumentary leishmaniasis
keywords:	Citometria de fluxo Ensaio de imunofluorescência indireta IgG L. ( V. ) braziliensis Leishmaniose tegumentar americana
DeCS:	Adolescente Adulto Envelhecido Anticorpos, Protozoários / sangue Anticorpos, protozoários / imunologia Fêmea Citometria de Fluxo / métodos Técnica de Anticorpo Fluorescente Indireta / métodos * Humanos Imunoglobulina G / imunologia Imunoglobulina G / uso terapêutico Leishmania braziliensis / imunologia Leishmaniose cutânea / sangue Leishmaniose cutânea / quimioterapia Leishmaniose cutânea / imunologia Masculino Meia idade Avaliação de Resultados (Cuidados de Saúde) / métodos Reprodutibilidade dos Resultados Fatores de Tempo Adulto jovem
Issue Date:	2013
Citation:	DE OLIVEIRA, A. P. et al. Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G. Journal of Immunological Methods, v. 387, n. 1–2, p. 245–253, 31 jan. 2013.
DOI:	10.1016/j.jim.2012.11.002
ISSN:	1872-7905
Copyright:	restricted access
Appears in Collections:	MG - IRR - Artigos de Periódicos PE - IAM - Artigos de Periódicos

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