Author | Morais, Rayana Carla Silva de | |
Author | Gonçalves, Suênia da Cunha | |
Author | Costa, Pietra Lemos | |
Author | Silva, Kamila Gaudêncio da | |
Author | Silva, Fernando José da | |
Author | Silva, Rômulo Pessoa E. | |
Author | Brito, Maria Edileuza Felinto de | |
Author | Brandão-Filho, Sinval Pinto | |
Author | Dantas-Torres, Filipe | |
Author | Paiva-Cavalcanti, Milena de | |
Access date | 2018-04-02T13:26:10Z | |
Available date | 2018-04-02T13:26:10Z | |
Document date | 2013 | |
Citation | DE MORAIS, R. C. S. et al. Detection of Leishmania infantum in animals and their ectoparasites by conventional PCR and real time PCR. Experimental & Applied Acarology, v. 59, n. 4, p. 473–481, abr. 2013. | pt_BR |
ISSN | 1572-9702 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25554 | |
Sponsorship | Graças à Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) (apoio técnico), Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ-PE) (apoio técnico, infra-estrutura e materiais de consumo) e Programa de Desenvolvimento Técnico de Insumos para a Saúde- PDTIS FIOCRUZ pelo uso de suas instalações. | pt_BR |
Language | eng | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Leishmania infantum | pt_BR |
Subject in Portuguese | Animais | pt_BR |
Subject in Portuguese | Ectoparasitas | pt_BR |
Subject in Portuguese | PCR em tempo real | pt_BR |
Subject in Portuguese | PCR | pt_BR |
Title | Detection of Leishmania infantum in animals and their ectoparasites by conventional PCR and real time PCR | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s10493-012-9611-4 | |
Abstract | Visceral leishmaniosis (VL) is a parasitic disease caused by Leishmania infantum, which is primarily transmitted by phlebotomine sandflies. However, there has been much speculation on the role of other arthropods in the transmission of VL. Thus, the aim of this study was to assess the presence of L. infantum in cats, dogs and their ectoparasites in a VL-endemic area in northeastern Brazil. DNA was extracted from blood samples and ectoparasites, tested by conventional PCR (cPCR) and quantitative real time PCR (qPCR) targeting the L. infantum kinetoplast DNA. A total of 280 blood samples (from five cats and 275 dogs) and 117 ectoparasites from dogs were collected. Animals were apparently healthy and not previously tested by serological or molecular diagnostic methods. Overall, 213 (76.1 %) animals and 51 (43.6 %) ectoparasites were positive to L. infantum, with mean parasite loads of 795.2, 31.9 and 9.1 fg in dogs, cats and ectoparasites, respectively. Concerning the positivity between dogs and their ectoparasites, 32 (15.3 %) positive dogs were parasitized by positive ectoparasites. The overall concordance between the PCR protocols used was 59.2 %, with qPCR being more efficient than cPCR; 34.1 % of all positive samples were exclusively positive by qPCR. The high number of positive animals and ectoparasites also indicates that they could serve as sentinels or indicators of the circulation of L. infantum in risk areas. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Federal Rural University of Pernambuco. Recife, PE, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Animals | pt_BR |
Subject | Ectoparasites | pt_BR |
Subject | PCR | pt_BR |
Subject | Real time PCR | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Brasil | pt_BR |
DeCS | Gatos | pt_BR |
DeCS | Ctenocefalides / parasitologia | pt_BR |
DeCS | Cães | pt_BR |
DeCS | Leishmania infantum / isolamento & purificação | pt_BR |
DeCS | Leishmaniose Visceral / transmissão | pt_BR |
DeCS | Phthiraptera / parasitologia | pt_BR |
DeCS | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
DeCS | Rhipicephalus sanguineus / parasitologia * | pt_BR |
Embargo date | 2050-01-01 | |