Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25554
Title: Detection of Leishmania infantum in animals and their ectoparasites by conventional PCR and real time PCR
Authors: Morais, Rayana Carla Silva de
Gonçalves, Suênia da Cunha
Costa, Pietra Lemos
Silva, Kamila Gaudêncio da
Silva, Fernando José da
Silva, Rômulo Pessoa E.
Brito, Maria Edileuza Felinto de
Brandão-Filho, Sinval Pinto
Dantas-Torres, Filipe
Paiva-Cavalcanti, Milena de
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Federal Rural University of Pernambuco. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: Visceral leishmaniosis (VL) is a parasitic disease caused by Leishmania infantum, which is primarily transmitted by phlebotomine sandflies. However, there has been much speculation on the role of other arthropods in the transmission of VL. Thus, the aim of this study was to assess the presence of L. infantum in cats, dogs and their ectoparasites in a VL-endemic area in northeastern Brazil. DNA was extracted from blood samples and ectoparasites, tested by conventional PCR (cPCR) and quantitative real time PCR (qPCR) targeting the L. infantum kinetoplast DNA. A total of 280 blood samples (from five cats and 275 dogs) and 117 ectoparasites from dogs were collected. Animals were apparently healthy and not previously tested by serological or molecular diagnostic methods. Overall, 213 (76.1 %) animals and 51 (43.6 %) ectoparasites were positive to L. infantum, with mean parasite loads of 795.2, 31.9 and 9.1 fg in dogs, cats and ectoparasites, respectively. Concerning the positivity between dogs and their ectoparasites, 32 (15.3 %) positive dogs were parasitized by positive ectoparasites. The overall concordance between the PCR protocols used was 59.2 %, with qPCR being more efficient than cPCR; 34.1 % of all positive samples were exclusively positive by qPCR. The high number of positive animals and ectoparasites also indicates that they could serve as sentinels or indicators of the circulation of L. infantum in risk areas.
Keywords: Leishmania infantum
Animals
Ectoparasites
PCR
Real time PCR
keywords: Leishmania infantum
Animais
Ectoparasitas
PCR em tempo real
PCR
DeCS: Animais
Brasil
Gatos
Ctenocefalides / parasitologia
Cães
Leishmania infantum / isolamento & purificação
Leishmaniose Visceral / transmissão
Phthiraptera / parasitologia
Reação em Cadeia da Polimerase em Tempo Real
Rhipicephalus sanguineus / parasitologia *
Issue Date: 2013
Citation: DE MORAIS, R. C. S. et al. Detection of Leishmania infantum in animals and their ectoparasites by conventional PCR and real time PCR. Experimental & Applied Acarology, v. 59, n. 4, p. 473–481, abr. 2013.
DOI: 10.1007/s10493-012-9611-4
ISSN: 1572-9702
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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