| Author | Solana, José Carlos | |
| Author | Ramírez, Laura | |
| Author | Corvo, Laura | |
| Author | Oliveira, Camila Indiani de | |
| Author | Barral Netto, Manoel | |
| Author | Requena, José María | |
| Author | Iborra, Salvador | |
| Author | Soto, Manuel | |
| Access date | 2018-04-05T18:56:36Z | |
| Available date | 2018-04-05T18:56:36Z | |
| Document date | 2017 | |
| Citation | SOLANA, J. C. et al. Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLoS Neglected Tropical Diseases, v. 11, n. 5, p. e0005644, 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25650 | |
| Sponsorship | Ministerio de Ciencia e Innovacion FISPI14/00366 (FEDER FUNDING) | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Leishmania major | pt_BR |
| Subject in Portuguese | Vacinas Atenuadas | pt_BR |
| Subject in Portuguese | Ratos endogâmicos | pt_BR |
| Subject in Portuguese | Animais | pt_BR |
| Title | Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0005644 | |
| Abstract | The immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings
The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously
and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection
was generated and parasites diminished progressively to become undetectable in most
of the analyzed organs. However, inoculation resulted in the long-term induction of parasite
specific IFN-γ responses able to control the disease caused by a challenge of L. major infective
promastigotes. BALB/c susceptible mice showed very low lesion development and a
drastic decrease in parasite burdens in the lymph nodes draining the site of infection and
internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to
the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection.
Conclusion/Significance
Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for
inducing moderate pro-inflammatory responses. These responses are able to confer longterm
protection in mice against the infection of L. major virulent parasites. | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain | pt_BR |
| Subject | Leismania infantum | pt_BR |
| Subject | Leishmania major | pt_BR |
| Subject | Vaccines, Attenuated | pt_BR |
| Subject | Mice, Inbred | pt_BR |
| Subject | Animals | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
