Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25650
Title: Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models
Authors: Solana, José Carlos
Ramírez, Laura
Corvo, Laura
Oliveira, Camila Indiani de
Barral Netto, Manoel
Requena, José María
Iborra, Salvador
Soto, Manuel
Affilliation: Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Universidad Autonoma de Madrid. Centro de Biologia Molecular Severo Ochoa. Departamento de Biologia Molecular. Nicolas Cabrera. Madrid, Spain
Abstract: The immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection was generated and parasites diminished progressively to become undetectable in most of the analyzed organs. However, inoculation resulted in the long-term induction of parasite specific IFN-γ responses able to control the disease caused by a challenge of L. major infective promastigotes. BALB/c susceptible mice showed very low lesion development and a drastic decrease in parasite burdens in the lymph nodes draining the site of infection and internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection. Conclusion/Significance Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for inducing moderate pro-inflammatory responses. These responses are able to confer longterm protection in mice against the infection of L. major virulent parasites.
Keywords: Leismania infantum
Leishmania major
Vaccines, Attenuated
Mice, Inbred
Animals
keywords: Leishmania infantum
Leishmania major
Vacinas Atenuadas
Ratos endogâmicos
Animais
Issue Date: 2017
Publisher: Public Library of Science
Citation: SOLANA, J. C. et al. Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLoS Neglected Tropical Diseases, v. 11, n. 5, p. e0005644, 2017.
DOI: 10.1371/journal.pntd.0005644
ISSN: 1935-2727
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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