Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25731
Title: kDNA minicircle signatures of Leishmania (Viannia) braziliensis in oral and nasal mucosa from mucosal leishmaniasis patients
Authors: Oliveira, Fernanda Santos de
Rosalino, Cláudia Maria Valete
Schubach, Armando de Oliveira
Pacheco, Raquel da Silva
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Otorrinolaringologia e Oftalmologia. Ri de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ. Brasil.
Abstract: Polymerase chain reaction (PCR) and low-stringency single-specific primer PCR (LSSP-PCR) analyses were used to detect Leishmania (Viannia) braziliensis DNA and investigate kDNA signatures of parasite populations present in oral and nasal mucosa lesions from mucosal leishmaniasis patients. A total of 25 samples from 22 patients were processed by specific PCR/hybridization assays. Parasite DNA was detected in all samples analyzed. The intraspecific polymorphism of the variable region of L. (V.) braziliensis kDNA minicircles was also investigated by LSSP-PCR. Similar kDNA signatures were observed in parasites recovered from nasal and oral mucosa lesions of the same patient. In contrast, genetically divergent profiles were detected in lesions from patients biopsied at different times within a period of 1 year. This is the first work to report genetic typing of L. (V.) braziliensis directly from human oral and nasal mucosal lesions.
Keywords: Mucosal leishmaniasis
Leishmania (Viannia) braziliensis
PCR/hybridization
LSSP-PCR
keywords: Leishmaniose mucosa
Reação em Cadeia da Polimerase
hibridização
Leishmania braziliensis
Issue Date: 2010
Publisher: Elsevier
Citation: OLIVEIRA, Fernanda Santos de; et al. kDNA minicircle signatures of Leishmania (Viannia) braziliensis in oral and nasal mucosa from mucosal leishmaniasis patients. Diagnostic Microbiology and Infectious Disease, v.66, p.361–365, 2010.
DOI: 10.1016/j.diagmicrobio.2009.11.004
ISSN: 0732-8893
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos
INI - Artigos de Periódicos

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