Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25836
Title: Antigenic fractions of Leishmania (Viannia) braziliensis: the immune response characterization of patients at the initial phase of disease
Authors: Brelaz, M. C. A.
Oliveira, A. P. de
Almeida, A. F. de
Souza, M. de Assis
Medeiros, Â. C. R.
Brito, M. E. F. de
Pereira, V. R. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade de Pernambuco. Departamento de Dermatologia. Recife, PE, Brasil
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: American cutaneous leishmaniasis (ACL) has different clinical manifestations and these manifestations are dependent on the immunological status of the host. As CD4(+) and CD8(+) T cells and their mediators play a fundamental role in the host response to Leishmania and there is also a search for antigenic molecules to be used as future vaccines and tools for prognostic tests, this study characterized ACL patients' immune response after stimulation with soluble and insoluble fractions of L. (V.) braziliensis. We demonstrated a prevailing production of the Th2 cytokines, IL-4 and IL-10 and a specific production of IFN-γ and TNF-α in patients before treatment. There was also a predominance of CD4(+) T cells and a small percentage CD8(+) T cells. The insoluble antigenic fraction primarily stimulated CD4(+) T cells, while the soluble antigenic fraction showed a mixed profile, with CD4(+) T cells being the main responsible for Th2 cytokines and CD8(+) T cells for Th1 cytokines. Therefore, our results showed that a down-modulation of the Th1 type of response occurs in the initial phase of L. braziliensis disease, being the antigenic fractions capable of stimulating a specific immune response.
Keywords: American cutaneous leishmaniasis
cytokines
flow cytometry
immune response
keywords: Leishmaniose cutânea americana
citocinas
citometria de fluxo
resposta imune
DeCS: Adolescente
Adulto
Envelhecido
Idoso, 80 anos ou mais
Antígenos, protozoários / química
Antígenos, protozoários / imunologia
Linfócitos T CD4-Positivos / imunologia
Citocinas / metabolismo
Fêmea
Citometria de Fluxo
Humanos
Interleucina-10 / metabolismo
Interleucina-4 / metabolismo
Leishmania braziliensis / imunologia
Leishmania braziliensis / patogenicidade
Leishmaniose cutânea / imunologia
Leishmaniose cutânea / parasitologia
Leishmaniose Cutânea / fisiopatologia
Masculino
Meia idade
Células Th2 / imunologia
Fatores de Tempo
Adulto jovem
Issue Date: 2012
Citation: BRELAZ, M. C. A. et al. Antigenic fractions of Leishmania (Viannia) braziliensis: the immune response characterization of patients at the initial phase of disease. Parasite Immunology, v. 34, n. 4, p. 236–239, abr. 2012.
DOI: 10.1111/j.1365-3024.2012.01351.x
ISSN: 1365-3024
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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