Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25851
Title: IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi
Authors: Melo, Adriene Siqueira de
Lorena, Virginia Maria Barros de
Braz, Suellen Carvalho de Moura
Docena, Cássia
Gomes, Yara de Miranda
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoparasitologia. Recife, PE, Brasil / Fundação Oswaldo Cruz/Fiocruz. Fiocruz Program for Research and Technological Development on Chagas Disease (PIDC). Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Núcleo de Plataformas Tecnológicas. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoparasitologia. Recife, PE, Brasil / Fundação Oswaldo Cruz/Fiocruz. Fiocruz Program for Research and Technological Development on Chagas Disease (PIDC). Rio de Janeiro, RJ, Brazil.
Abstract: Along with several other aspects of Chagas disease, the mechanisms responsible for the different clinical outcomes observed in chronic infected individuals have not yet been clarified. It is believed that the host immune response to the parasite plays an important role in the development of the pathology. Therefore, the aim of this study was to evaluate the relationship between IL-10 and IFN-γ gene expression profile, after in vitro stimulation of peripheral blood mononuclear cells (PBMC) with Trypanosoma cruzi recombinant antigens CRA (cytoplasmatic repetitive antigen) and FRA (flagellar repetitive antigen), and the clinical forms of chronic Chagas disease. Twenty patients with the cardiac form of the disease (CARD), of whom 10 had the mild cardiac form (CARD 1) and 10 the severe cardiac form (CARD 2), and 20 patients with the indeterminate form (IND), were selected at the Chagas Disease Unit of the Oswaldo Cruz University Hospital, University of Pernambuco, Recife, Pernambuco, Brazil. The PBMCs of these individuals were cultured in the presence of CRA or FRA for 3 days and IL-10 and IFN-γ gene expression was evaluated by detection of its messenger RNA using Real Time Quantitative PCR. Although no significant difference was observed between the groups of individuals studied, we found that most patients with IND displayed high levels of IFN-γ gene expression, while the majority of patients with CARD 1 presented high levels of IL-10. The results of this study thus highlight the important role that inflammatory cytokines play in patients with the IND group controlling for parasite replication, and that anti-inflammatory cytokines play in determining susceptibility to progression to symptomatic clinical forms of the disease.
Keywords: Chagas disease
Cytokines
Gene expression
Biological markers
Recombinant antigens
keywords: Doença de Chagas
Citocinas
Expressão genetica
Marcadores biológicos
Antígenos Recombinantes
DeCS: Adulto
Antígenos, protozoários / administração e dosagem
Seqüência Base
Doença de Chagas / genética
Doença crônica
Primers do DNA
Fêmea
Humanos
Técnicas In Vitro
Interferon-gama / genética
Interleucina-10 / genética
Masculino
Meia idade
Trypanosoma cruzi / genética
Trypanosoma cruzi / imunologia
Issue Date: 2012
Publisher: Elsevier
Citation: DE MELO, A. S. et al. IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi. Cytokine, v. 58, n. 2, p. 207–212, maio 2012.
DOI: 10.1016/j.cyto.2012.01.008
ISSN: 1096-0023
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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