| Author | Ribeiro, Mayara | |
| Author | Franco, Priscila Silva | |
| Author | Maria, Janice Buiate Lopes | |
| Author | Angeloni, Mariana Bodini | |
| Author | Barbosa, Bellisa de Freitas | |
| Author | Gomes, Angelica de Oliveira | |
| Author | Castro, Andressa Silva | |
| Author | Silva, Rafaela José da | |
| Author | Oliveira, Fernanda Chaves de | |
| Author | Milian, Iliana Claudia Balga | |
| Author | Martins Filho, Olindo Assis | |
| Author | Ietta, Francesca | |
| Author | Mineo, José Roberto | |
| Author | Ferro, Eloisa Amália Vieira | |
| Access date | 2018-04-17T19:36:05Z | |
| Available date | 2018-04-17T19:36:05Z | |
| Document date | 2017 | |
| Citation | RIBEIRO, Mayara et al. Azithromycin treatment is able to control the infection by two genotypes of Toxoplasma gondii in human trophoblast BeWo cells. Exp Parasitol., v. 181, p. 111-118, 2017 | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25918 | |
| Language | eng | pt_BR |
| Publisher | Academic Press | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | azitromicina | pt_BR |
| Subject in Portuguese | Toxoplasmose | pt_BR |
| Title | Azithromycin treatment is able to control the infection by two genotypes of Toxoplasma gondii in human trophoblast BeWo cells | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2017.08.004 | |
| Abstract | Trophoblast infection by Toxoplasma gondii plays a pivotal role in the vertical transmission of toxoplasmosis. Here, we investigate whether the antibiotic therapy with azithromycin, spiramycin and sulfadiazine/pyrimethamine are effective to control trophoblast infection by two Brazilian T. gondii genotypes, TgChBrUD1 or TgChBrUD2. Two antibiotic protocols were evaluated, as follow: i) pre-treatment of T. gondii-tachyzoites with selected antibiotics prior trophoblast infection and ii) post-treatment of infected trophoblasts. The infection index/replication and the impact of the antibiotic therapy on the cytokine milieu were characterized. It was observed that TgChBrUD2 infection induced lower infection index/replication as compared to TgChBrUD1. Regardless the therapeutic protocol, azithromycin was more effective to control the trophoblast infection with both genotypes when compared to conventional antibiotics. Azithromycin induced higher IL-12 production in TgChBrUD1-infected cells that may synergize the anti-parasitic effect. In contrast, the effectiveness of azithromycin to control the TgChBrUD2-infection was not associated with the IL-12 production. BeWo-trophoblasts display distinct susceptibility to T. gondii genotypes and the azithromycin treatment showed to be more effective than conventional antibiotics to control the T. gondii infection/replication regardless the parasite genotype | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciencias Biologicas. Departamento de Biologia Estrutural. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of Siena. Department of Life Science. Aldo Moro Road 2, Siena, Italy | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Imunologia, Microbiologia e Parasitologia. Laboratorio de Imunoparasitologia. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil | pt_BR |
| Subject | Trophoblast cell | pt_BR |
| Subject | Azithromycin | pt_BR |
| Subject | Toxoplasma gondii atypical strains | pt_BR |
| Subject | Cytokines | pt_BR |
| Embargo date | 2022-01-01 | |
