Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25918
Title: Azithromycin treatment is able to control the infection by two genotypes of Toxoplasma gondii in human trophoblast BeWo cells
Authors: Ribeiro, Mayara
Franco, Priscila Silva
Maria, Janice Buiate Lopes
Angeloni, Mariana Bodini
Barbosa, Bellisa de Freitas
Gomes, Angelica de Oliveira
Castro, Andressa Silva
Silva, Rafaela José da
Oliveira, Fernanda Chaves de
Milian, Iliana Claudia Balga
Martins Filho, Olindo Assis
Ietta, Francesca
Mineo, José Roberto
Ferro, Eloisa Amália Vieira
Affilliation: Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciencias Biologicas. Departamento de Biologia Estrutural. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil
University of Siena. Department of Life Science. Aldo Moro Road 2, Siena, Italy
Universidade Federal de Uberlândia. Departamento de Imunologia, Microbiologia e Parasitologia. Laboratorio de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Departamento de Histologia e Embriologia. Laboratorio de Imunofisiologia Reprodutiva. Uberlândia, MG, Brazil
Abstract: Trophoblast infection by Toxoplasma gondii plays a pivotal role in the vertical transmission of toxoplasmosis. Here, we investigate whether the antibiotic therapy with azithromycin, spiramycin and sulfadiazine/pyrimethamine are effective to control trophoblast infection by two Brazilian T. gondii genotypes, TgChBrUD1 or TgChBrUD2. Two antibiotic protocols were evaluated, as follow: i) pre-treatment of T. gondii-tachyzoites with selected antibiotics prior trophoblast infection and ii) post-treatment of infected trophoblasts. The infection index/replication and the impact of the antibiotic therapy on the cytokine milieu were characterized. It was observed that TgChBrUD2 infection induced lower infection index/replication as compared to TgChBrUD1. Regardless the therapeutic protocol, azithromycin was more effective to control the trophoblast infection with both genotypes when compared to conventional antibiotics. Azithromycin induced higher IL-12 production in TgChBrUD1-infected cells that may synergize the anti-parasitic effect. In contrast, the effectiveness of azithromycin to control the TgChBrUD2-infection was not associated with the IL-12 production. BeWo-trophoblasts display distinct susceptibility to T. gondii genotypes and the azithromycin treatment showed to be more effective than conventional antibiotics to control the T. gondii infection/replication regardless the parasite genotype
Keywords: Trophoblast cell
Azithromycin
Toxoplasma gondii atypical strains
Cytokines
keywords: azitromicina
Toxoplasmose
Issue Date: 2017
Publisher: Academic Press
Citation: RIBEIRO, Mayara et al. Azithromycin treatment is able to control the infection by two genotypes of Toxoplasma gondii in human trophoblast BeWo cells. Exp Parasitol., v. 181, p. 111-118, 2017
DOI: 10.1016/j.exppara.2017.08.004
ISSN: 0014-4894
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.