Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26056
Title: Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
Authors: Cruz, Heidi Lacerda Alves da
Montenegro, Rosana de Albuquerque
Lima, Juliana Falcão de Araújo
Poroca, Diogo da Rocha
Lima, Juliana Figueirêdo da Costa
Montenegro, Lílian Maria Lapa
Crovella, Sergio
Schindler, Haiana Charifker
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil / University of Trieste. Genetic Service IRCCS Burlo Garofolo. Trieste, Italy.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.
Keywords: Mycobacterium tuberculosis
Nested-PCR
Blood
Urine
IS6110 insertion sequence
Molecular diagnosis
keywords: Mycobacterium tuberculosis
Nested-PCR
Sangue
Urina
Diagnóstico molecular
Sequência de inserção IS6110
DeCS: Reação em Cadeia da Polimerase
nested-PCR
Tuberculose / diagnóstico
Tuberculose / sangue
Coleta de Amostras Sanguíneas
Tuberculose / transmissão
Brasil / epidemiologia
Humanos
Sensibilidade e especificidade
urina
Issue Date: 2011
Citation: DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011.
DOI: 10.1590/S1517-83822011000100041
ISSN: 1517-8382
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos

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