Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26085
Title: Cytokine levels in serious cardiopathy of Chagas disease after in vitro stimulation with recombinant antigens from Trypanosoma cruzi
Authors: Lorena, V. M. B.
Lorena, I. M. B.
Braz, S. C. M.
Melo, A. S.
Melo, M. F. A. D.
Melo, M. G. A. C.
Silva, E. D.
Ferreira, A. G. P.
Morais, C. N. L.
Costa, V. M. A.
Correa-Oliveira, R.
Gomes, Y. M.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Universidade de Pernambuco. Hospital Universitário Oswaldo Cruz. Recife, PE, Brazil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Universidade Federal de Pernambuco. Departamento de Imunologia do LIKA. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Universidade Federal de Pernambuco. Departamento de Imunologia do LIKA. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Reativos para Diagnóstico. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas Renè Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Program for Research and technological Development on Chagas Disease. Rio de Janeiro, RJ, Brazil.
Abstract: The clinical manifestations of human Chagas disease are associated with distinct and complex host-parasite interactions that directly involve the host's immune system. In this study, we analysed the relationship between the production of intracytoplasmic cytokines after in vitro stimulation with the recombinant antigens CRA (cytoplasmatic repetitive antigen) or FRA (flagellar repetitive antigen) from Trypanosoma cruzi and the chronic cardiac or indeterminate clinical forms of Chagas disease. The chagasic patient groups consisted of 39 individuals, selected at the Chagas Disease Unit of the Oswaldo Cruz University Hospital, whom presented either a cardiac form without cardiac dilatation (CARD 1), cardiac form with cardiac dilatation (CARD 2) or indeterminate form (IND). Blood samples were obtained from these patients and cultured in the presence of CRA or FRA. The cytokines produced by lymphocytes and monocytes after antigen stimulation were analysed by flow cytometry. Our results showed that the IFN-γ and TNF-α, produced by CD8+ T lymphocytes after in vitro stimulation with CRA, differed among chagasic patients with CARD 1, CARD 2 or IND. We propose that these cytokines could be utilized as immunological markers for clinical cardiac forms of Chagas disease. In a prospective study of patients presenting IND and CARD 1, the assay performed in this paper could serve as a tool to monitor therapeutic interventions, thus improving the patient's quality of life.
Keywords: Chagas disease
Cytokines
Trypanosoma cruzi
Flow cytometry
keywords: Doença de Chagas
Citocinas
Trypanosoma cruzi
Citometria de fluxo
DeCS: Adulto
Envelhecido
Antígenos, protozoários / imunologia
Biomarcadores / metabolismo
Células Cultivadas
Cardiomiopatia Chagásica / diagnóstico
Cardiomiopatia Chagásica / imunologia
Citocinas / biossíntese
Progressão da doença
Fêmea
Flagelo / imunologia
Humanos
Linfócitos / imunologia
Masculino
Meia idade
Monócitos / imunologia
Proteínas Recombinantes / imunologia
Trypanosoma cruzi / imunologia
Issue Date: 2010
Citation: LORENA, V. M. B. et al. Cytokine levels in serious cardiopathy of Chagas disease after in vitro stimulation with recombinant antigens from Trypanosoma cruzi. Scandinavian Journal of Immunology, v. 72, n. 6, p. 529–539, Dec. 2010.
DOI: 10.1111/j.1365-3083.2010.02462.x
ISSN: 1365-3083
Copyright: restricted access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
MG - IRR - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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