| Author | Melo, Cristiane Moutinho Lagos de | |
| Author | Castro, Maria Carolina Accioly Brelaz de | |
| Author | Oliveira, Andresa Pereira de | |
| Author | Gomes, Fabiana Oliveira Santos | |
| Author | Pereira, Valéria Rêgo Alves | |
| Author | Correia, Maria Tereza Santos | |
| Author | Coelho, Luana Cassandra Breitenbach Barroso | |
| Author | Paiva, Patrícia Maria Guedes | |
| Access date | 2018-04-25T15:06:22Z | |
| Available date | 2018-04-25T15:06:22Z | |
| Document date | 2010 | |
| Citation | DE MELO, C. M. L. et al. Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes. Phytotherapy research: PTR, v. 24, n. 11, p. 1631–1636, nov. 2010. | pt_BR |
| ISSN | 1099-1573 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26093 | |
| Sponsorship | Conselho Nacional de Pesquisa (CNPq), Coordenação de Estudos Avançados em Educação Superior (CAPES) e Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, em Recife (CPqAM / FIOCRUZ). | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Imunomodulação | pt_BR |
| Subject in Portuguese | Lectina | pt_BR |
| Subject in Portuguese | Cratylia mollis | pt_BR |
| Subject in Portuguese | Cramoll 1,4 | pt_BR |
| Subject in Portuguese | Óxido nítrico | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Title | Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes | pt_BR |
| Type | Article | |
| DOI | 10.1002/ptr.3156 | |
| Abstract | Cramoll 1,4 is a lectin extracted from Cratylia mollis Mart. seeds that has shown antitumor and lymphocyte mitogenic activities in other studies. The aim of this work was to investigate, in vitro, the immunomodulatory activity of Cramoll 1,4 on experimental cultures of mice lymphocytes through cytotoxic assays, nitric oxide (NO) concentrations and IL-10 and IFN-γ production. Cramoll 1,4 did not show cytotoxic activity at 1-25 μg/mL concentrations, similar results were observed with concanavalin A (Con A) and phytohemagglutinin (PHA) lectins. The minimum production of IL-10 was observed in splenocytes cultivated with Con A, PHA and Cramoll 1,4 lectins. However, splenocytes treated with Cramoll 1,4 showed higher IFN-γ production in comparison with PHA and Con A (p < 0.05 for both). Production of NO was effectively suppressed in murine cells stimulated with the lectins and was only detected after 72 h for PHA in relation to non-stimulated lymphocytes (p < 0.05). Cramoll 1,4 was not toxic to murine lymphocytes, induced Th1 response through IFN-γ production and showed antiinflammatory activity through NO suppression. Therefore, Cramoll 1,4 can be considered a lectin with immunomodulatory activity. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Concanavalina A / farmacologia | pt_BR |
| DeCS | Fabaceae / química | pt_BR |
| DeCS | Imunomodulação | pt_BR |
| DeCS | Interferona-gama / imunologia | pt_BR |
| DeCS | Interleucina-10 / imunologia | pt_BR |
| DeCS | Lectinas / farmacologia | pt_BR |
| DeCS | Linfócitos / efeitos de drogas | pt_BR |
| DeCS | Linfócitos / imunologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Ratos | pt_BR |
| DeCS | Ratos, endogâmicos BALB C | pt_BR |
| DeCS | Óxido nítrico / metabolismo | pt_BR |
| DeCS | Fitohemaglutininas / farmacologia | pt_BR |
| DeCS | Sementes / química | pt_BR |
| DeCS | Baço / citologia | pt_BR |
| Embargo date | 2050-01-01 | |
