Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26093
Title: Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes
Authors: Melo, Cristiane Moutinho Lagos de
Castro, Maria Carolina Accioly Brelaz de
Oliveira, Andresa Pereira de
Gomes, Fabiana Oliveira Santos
Pereira, Valéria Rêgo Alves
Correia, Maria Tereza Santos
Coelho, Luana Cassandra Breitenbach Barroso
Paiva, Patrícia Maria Guedes
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Departamento de Bioquímica. Recife, PE, Brasil.
Abstract: Cramoll 1,4 is a lectin extracted from Cratylia mollis Mart. seeds that has shown antitumor and lymphocyte mitogenic activities in other studies. The aim of this work was to investigate, in vitro, the immunomodulatory activity of Cramoll 1,4 on experimental cultures of mice lymphocytes through cytotoxic assays, nitric oxide (NO) concentrations and IL-10 and IFN-γ production. Cramoll 1,4 did not show cytotoxic activity at 1-25 μg/mL concentrations, similar results were observed with concanavalin A (Con A) and phytohemagglutinin (PHA) lectins. The minimum production of IL-10 was observed in splenocytes cultivated with Con A, PHA and Cramoll 1,4 lectins. However, splenocytes treated with Cramoll 1,4 showed higher IFN-γ production in comparison with PHA and Con A (p < 0.05 for both). Production of NO was effectively suppressed in murine cells stimulated with the lectins and was only detected after 72 h for PHA in relation to non-stimulated lymphocytes (p < 0.05). Cramoll 1,4 was not toxic to murine lymphocytes, induced Th1 response through IFN-γ production and showed antiinflammatory activity through NO suppression. Therefore, Cramoll 1,4 can be considered a lectin with immunomodulatory activity.
keywords: Imunomodulação
Lectina
Cratylia mollis
Cramoll 1,4
Óxido nítrico
Citocinas
DeCS: Animais
Células Cultivadas
Concanavalina A / farmacologia
Fabaceae / química
Imunomodulação
Interferona-gama / imunologia
Interleucina-10 / imunologia
Lectinas / farmacologia
Linfócitos / efeitos de drogas
Linfócitos / imunologia
Masculino
Ratos
Ratos, endogâmicos BALB C
Óxido nítrico / metabolismo
Fitohemaglutininas / farmacologia
Sementes / química
Baço / citologia
Issue Date: 2010
Citation: DE MELO, C. M. L. et al. Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes. Phytotherapy research: PTR, v. 24, n. 11, p. 1631–1636, nov. 2010.
DOI: 10.1002/ptr.3156
ISSN: 1099-1573
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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