Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26380
Title: T-lymphocytes in experimental Leishmania amazonensis infection: comparison between immunized and naive BALB/c mice
Authors: Pompeu, Margarida
Freitas, A. L
Reis, G. A. dos
Barral Netto, Manoel
Affilliation: Universidade Federal da Bahia. Faculdade de Medicina. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Medicina. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brasil
Universidade Feederal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil
Universidade Federal da Bahia. Faculdade de Medicina. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brasil
Abstract: Abstract. Highly susceptible naive BALB/c mice or mice that had previously been immunized i.v. with solubilized homologous antigen (partially resistant) were infected with Leishmania amazonensis. Histologically, the main differences between the two groups were lymphocytic infiltration and macrophage activation. Assays of T-cell function at 3 and 10 weeks after infection revealed that purified T-cells did not proliferate following treatment with leishmania antigen. A mitogenic anti-CD3 monoclonal antibody (mAb) failed to activate T-cells after 3 weeks of infection as judged by proliferation and IL-2 secretion assays. After 10 weeks of infection, anti-CD3 mAb fully activated T-cells to proliferation and IL-2 secretion. On the other hand, T-cells released IL-3 in response to leishmania antigen, anti-CD3 mAb and anti- Thyl mAb at 3 and 10 weeks post-infection. Surprisingly, a mitogenic anti-Thy 1 mAb (G7) fully activated Tcells even at 3 weeks of infection as judged by proliferative and IL-2 secretion assays. No significant differences were found in the proliferative or interleukin secretory responses of T-cells from animals that had been infected in either the presence or the absence of prior immunization. Since the Thyl triggering pathway has different accessory cell and cytokine requirements than does the CD3 : TCR lymphocyte activation pathway, it is possible that immunization was more effective in changing the cellular interactions of the T-lymphocyte than in altering its intrinsic capabilities.
Keywords: Leishmania amazonensis
T-lymphocytes
Infection
Animals
Mice
keywords: Leishmania amazonensis
Linfócitos T
Infecção
Animais
Camundongos
Issue Date: 1992
Publisher: Springer Verlag
Citation: POMPEU, M. et al. T-lymphocytes in experimental Leishmania amazonensis infection: comparison between immunized and naive BALB/c mice. Parasitology Research, v. 78, p. 16-22, 1992.
Description: Barral Netto, Manoel. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
ISSN: 0932-0113
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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