Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26449
Title: Collectins and galectins in the abomasum of goats susceptible and resistant to gastrointestinal nematode infection
Authors: Souza, Bárbara Maria Paraná da Silva
Lamberta, Sabrina Mota
Nishia, Sandra M
Saldaña, Gustavo F
Oliveira, Geraldo Gileno de Sá
Vieira, Luis S
Madruga, Claudio R
Almeida, Maria Angela Ornelas de
Affilliation: Federal University of Bahia. School of Veterinary Medicine and Animal Science. Laboratory of Cellular and Molecular Biology. Salvador, BA, Brazil
Federal University of Bahia. School of Veterinary Medicine and Animal Science. Laboratory of Cellular and Molecular Biology. Salvador, BA, Brazil
Federal University of Bahia. School of Veterinary Medicine and Animal Science. Laboratory of Cellular and Molecular Biology. Salvador, BA, Brazil
Institute for Research on Genetic Engineering and Molecular Biology. Laboratory of Molecular Biology of Chagas Disease. Buenos Aires, Argentina
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunologia Celular e Molecular. Salvador, BA, Brasil
National Research Center of Goats and Sheep. Embrapa. Sobral, CE, Brazil
Federal University of Bahia. School of Veterinary Medicine and Animal Science. Laboratory of Cellular and Molecular Biology. Salvador, BA, Brazil
Federal University of Bahia. School of Veterinary Medicine and Animal Science. Laboratory of Cellular and Molecular Biology. Salvador, BA, Brazil
Abstract: Originally described in cattle, conglutinin belongs to the collectin family and is involved in innate immune defense. It is thought that conglutinin provides the first line of defense by maintaining a symbiotic relationship with the microbes in the rumen while inhibiting inflammatory reactions caused by antibodies leaking into the bloodstream. Due to the lack of information on the similar lectins and sequence detection in goats, we characterized the goat conglutinin gene using RACE and evaluated the differences in its gene expression profile, as well as in the gene expression profiles for surfactant protein A, galectins 14 and 11, interleukin 4 and interferongamma in goats. We used Saanen and Anglo Nubian F2 crossbred goats monitored over a period of four months and characterized them as resistant (R) or susceptible (S) based on the average values of EPG counts. Goat conglutinin was similar to bovine conglutinin, but its gene expression varied among different tissues. However, as with bovine conglutinin, it was most highly expressed in the liver. Variation in conglutinin (R=24.3 ± 3.9; S =23.5 ± 2.6, p=0.059), protein surfactant A (R=23.8 ± 5.2, S =24.4 ± 2.3, p =0.16), galectin 14 (R =15.9 ± 3.5, S=14.7 ± 6.2, p =0.49) and galectin l1 gene expression (R=25.4 ± 2.6, S =25.8 ± 3.7, p =0.53) was not significant between groups. However, there were weak correlations between interleukin 4 and the protein surfactant A gene (r=0.459, p= 0.02) and between interleukin 4 and galectin 11 (r= 0.498, p =0.01). Strong correlation between interferon-gamma and galectin 14 (r =0.744, p =0.00) was observed. Galectin 14 was negatively correlated with the number of nematodes in the goat (r =−0.416, p=0.04) as well as the EPG count (r =−0.408, p =0.04). This is the first study to date that identifies the gene expression of conglutinin, surfactant protein A and galectins 14 and 11 in the goat abomasum. In conclusion, we present evidence that lectin is involved in the immune response to gastrointestinal nematodes, which suggests that collectins and galectins are involved in the molecular recognition of helminths.
Keywords: Innate immunity
Lectins
Helminth
Ruminants
PCR
keywords: Imunidade inata
Lectinas
Helmintos
Ruminantes
PCR
Issue Date: 2018
Publisher: Elsevier
Citation: SOUZA, B. M. P. S. et al. Collectins and galectins in the abomasum of goats susceptible and resistant to gastrointestinal nematode infection. Veterinary Parasitology: Regional Studies and Reports, v. 12, p. 99–105, 2018.
ISSN: 2405-9390
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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