Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26532
Title: Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection
Authors: Alessio, Glaucia Diniz
Araujo, Fernanda Fortes de
Cortes, Denise Fonseca
Sales Junior, Policarpo Ademar
Lima, Daniela Cristina
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antonio Pascoal
Carvalho, Andrea Teixeira de
Martins Filho, Olindo Assis
Lana, Marta de
Affilliation: Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doenças Infecciosas e Parasitarias. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
Abstract: Distinct Trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of Chagas disease. However, typing strategies for genotype- specific serodiagnosis of Chagas disease are still unavailable and requires standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas Flow ATE-IgG2a technique was developed with applicability for universal and genotypespecific diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples (percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel batches of TcI, TcVI and TcII target antigens. Data demonstrated that ªα-TcII-TRYPO/ 1:500, cut-off/PPFP = 20%º presented an excellent performance for universal diagnosis of T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes ªα-TcITRYPO/ 1:4,000, cut-off/PPFP = 50%º, ªα-TcII-AMA/1:1,000, cut-off/PPFP = 40%º and ªα- TcVI-EPI/1:1,000, cut-off/PPFP = 45%º showed good performance to segregate infections with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana and TcII/Y strains displayed opposite patterns of reactivity with ªα-TcI TRYPOº and ªα-TcII AMAº. Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern. The method presented a good performance for genotype-specific diagnosis, with global accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections and 94% when comprise only TcI and TcII infections. This study also proposes a receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for universal and genotype-specific diagnosis of T. cruzi infection.
Keywords: Trypanosoma cruzi
Chagas disease
keywords: Trypanosoma cruzi
Doença de Chagas
Issue Date: 2017
Publisher: Public Library of Science
Citation: ALESSIO, Glaucia Diniz et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 2017
DOI: 10.1371/journal.pntd.0005444
ISSN: 1935-2727
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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