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THE DAU ALLELE AND ANTI-D ALLOIMMUNIZATION PRESENT WITH HIGH FREQUENCY IN BRAZILIAN SICKLE CELL DISEASE PATIENTS
Author
Affilliation
Universidade Federal do Amazonas. Faculdade de Farmacia. Manaus, AM, Brasil
Universidade do Estado da Bahia. Departamento de Ciencias da Vida. Salvador, BA, Brasil
Universidade Federal do Amazonas. Faculdade de Farmacia. Manaus, AM, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia, Salvador, BA, Brasil
Universidade do Estado da Bahia. Departamento de Ciencias da Vida. Salvador, BA, Brasil
Universidade Federal do Amazonas. Faculdade de Farmacia. Manaus, AM, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia, Salvador, BA, Brasil
Abstract
Background: Antigens DIIIa, DAR and DAU are common in people
of African descent and are involved in anti-D alloimmunization.
Sickle cell disease (SCD) patients frequently need blood therapy and
are vulnerable to alloimmunization.
Methods: The study included SCD patients from the Brazilian state
of Bahia, which has the highest incidence of the disease in Brazil; 241
SCD patients and 220 healthy individuals were studied. Alleles were
characterized by PCR-RFLP and PCR-SSP techniques.
Results: The DAU allele was found in 22.3% (43/193) of the SCD
patients. Two (1%) patients had the DIIIa/D wild-type genotype, one
(0.5%) had the DIIIa/D- genotype, 11 (5.7%) had the DAR/D wildtype
genotype and three (1.6%) had the DAR/D- genotype. Two patients
were positive for the 667T>G mutation and the 1136C>T mutation,
one (0.5%) had the genotype DIIIa/DAU, and one (0.5%) had
the genotype DAR/DAU.
Conclusion: There was statistical significance when the allele frequencies
were evaluated among SCD, sickle cell anemia (HbSS)
patients and healthy individuals. The frequencies of the DIIIa, DAR
and DAU alleles among SCD patients differ from those of healthy
individuals from the same population, and a high frequency of the
DAU variant was associated with anti-D alloimmunization in these
patients.
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