Objectives: To evaluate the correlation of the total distance walked during the six-minute walk test (6MWT) with left ventricular function and quality of life in patients with Chagas Disease (ChD) complicated by heart failure. Methods: This is a cross-sectional study of adult patients with ChD and heart failure diagnosed based on Framingham criteria. 6MWT was performed following international guidelines. New York Heart Association functional class, brain natriuretic peptide (BNP) serum levels, echocardiographic parameters and quality of life (SF-36 and MLHFQ questionnaires) were determined and their correlation with the distance covered at the 6MWT was tested. Results: Forty adult patients (19 male; 60 12 years old) with ChD and heart failure were included in this study. The mean left ventricular ejection fraction was 35 12%. Only two patients (5%) ceased walking before 6 min had elapsed. There were no cardiac events during the test. The average distance covered was 337 105 metres. The distance covered presented a negative correlation with BNP (r = 0.37; P = 0.02), MLHFQ quality-of-life score (r = 0.54; P = 0.002), pulmonary artery systolic pressure (r = 0.42; P = 0.02) and the degree of diastolic dysfunction (r = 0.36; P = 0.03) and mitral regurgitation (r = 0.53; P = 0.0006) and positive correlation with several domains of the SF-36 questionnaire. Conclusions: The distance walked during the 6MWT correlates with BNP, quality of life and parameters of left ventricular diastolic function in ChD patients with heart failure. We propose this test to be adopted in endemic areas with limited resources to aid in the identification of patients who need referral for tertiary centres for further evaluation and treatment.